检测T-2毒素的直接竞争性酶联免疫吸附测定法的研究  

Establishment of the monoclonal antibody based-enzyme-linked immunosorbent assay for the detection of T-2 toxin in cereals

作　　者：曹艳红[1] 孟宪清[1] 冯杰[1] 惠洋[1] 孙殿军[1] 

机构地区：[1]哈尔滨医科大学地方病控制中心大骨节病研究所,哈尔滨150086

出　　处：《中国人兽共患病学报》2006年第2期132-135,共4页Chinese Journal of Zoonoses

基　　金：黑龙江省科技攻关项目(G00C191801);黑龙江省杰出青年科学基金项目(J01-04);黑龙江省卫生厅资助项目(2003-118)

摘　　要：目的 本研究在自制T-2毒素酶标半抗原的基础上，结合市售抗T-2毒素单克隆抗体，建立了检测谷物中T-2毒素的直接竞争性酶联免疫吸附测定法（ELISA）。方法粮食样品经70％甲醇-水溶液提取，用滤纸过滤并稀释后。再用0．45μm滤器过滤净化，该滤液用于ELISA检测。结果本方法最低检出量为0．125ng／ml，添加70-280ng／g T-2毒素的大米样品和面粉样品的平均回收率分别为85～117．5％和98．1～102．5％。结论结果表明，该方法简化了粮样提取步骤。可用于谷物及加工产品中T-2毒素的检测。A monoclonal antibody based-direct competitive enzyme linked immunoabsorbent assay （ELISA） was developed for the quantitative determination of T-2 toxin in cereals in the present study, in which 70 % of methanol-water solution was used to extract the cereal samples; The extracts filtered through Whatman No. 4 filter paper and diluted, were clarified by passing through the filter with 0.45 μm pores, and this purified extract was used in the detection of T-2 toxin by ELISA. It was found that the minimal amount of toxin to be detected by this method was 0. 125 ng/ml, and the recovery rates of the rice and flour samples spiked with 70-280 tag of T-2 toxin per gram were 92.3 % -112.7 % and 98.8% -102.5 % respectively. It is evident that this method of testing is proved to be suitable for the screening of T-2 toxin in cereals.

关 键 词：T-2毒素 直接竞争性酶联免疫吸附测定 谷物 

分 类 号：R379[医药卫生—病原生物学]