Rh血型DEL表型RhD蛋白表达的研究  

Research of RhD protein in Rh blood group Delphenotype

作　　者：熊文[1] 邵超鹏[1] 李雪梅[1] 周一炎[1] 

机构地区：[1]深圳市血液中心输血医学研究所,518035

出　　处：《中华医学杂志》2006年第2期128-132,共5页National Medical Journal of China

摘　　要：目的探讨Del血型个体是否表达正常的RhD蛋白。方法采用逆转录-聚合酶链反应(RT-PCR)技术和cDNA序列分析技术,检测不同Rh表型Del血型(CCDelDelee、CcDeldee和CcDelDelee)个体的RhD mRNA,分析Del蛋白的表达。结果Del血型个体有复杂的RhD mRNA形式,检测到缺失第7至第9外显子(Del789)、缺失第7和第9外显子(Del79)、缺失第8和第9外显子(Del89)、缺失第9外显子(Del9)、缺失第8和第9外显子加第7内含子的170个碱基(Del89+170)(917-1086,Genbank AB035194)、缺失第9外显子加第7内含子的170个碱基(Del9+170)等共6种形式的RhD转录子。这些转录子均缺失第9外显子,其中转录子Del9与正常RhD mRNA最相似。结论Del血型不存在正常的RhD mRNA,不编码正常的RhD蛋白质。Objective To study the expression of RhD protein in Rh blood group Del phenotype. Methods Peripheral blood samples were collected from 3 voluntary blood donors with different Rh phenotypes ： CCD^el D^elee, CcD^eldee, and CcDelD^elee, and 4 controls： 2 RhD positive with the phenotypes of CCDDee and CcDDEe, and 2 RhD negative with the phenotypes： Ccddee and ccddee. Reverse transcriptase PCR （RT-PCR） and cDNA sequencing were used to detect the RhD mRNA and the expression of Del protein. Results The Del individuals all showed complicated RhD mRNA isolations, including 6 transcripts： isoforms with exons 7-9 spliced, exons 7 and 9 spliced, exons 8 and 9 spliced, exon 9 spliced and 2 long transcripts with exons 8 and 9 spliced or exon 9 spliced, but containing an additional segment of sequence from RHD intron 7 （917-1086, GenBank AB035194）, those transcripts all being out of exon 9 and the Del9 transcript being the most similar to the normal RhD mRNA. Conclusion Normal RhD mRNA does not exist in the Del blood group that does not code normal RhD protein.

关 键 词：RH-HR血型系统 蛋白质类 翻译 RNA 信使 DNA 互补 

分 类 号：R346[医药卫生—基础医学]