CDA-Ⅱ诱导人胶质瘤细胞分化与抑瘤的作用  

Effect of CDA-Ⅱ on the differentiation and inhibition of human glioma cell line SWO-38

作　　者：王红艳[1] 钟雪云[1] 涂永生[2] 刘致中[1] 

机构地区：[1]暨南大学医学院病理学教研室,广东广州510632 [2]广州医学院生理学教研室,广东广州510182

出　　处：《中国药理学通报》2006年第2期180-184,共5页Chinese Pharmacological Bulletin

基　　金：国家科技部重大基础研究前期研究专项"973"资助项目(No2002ccc0400);高等学校博士学科点专项科研基金资助项目(No20040559008);广东省科技厅研究团队资助项目(No015012);广州市科技局科研重点立项项目(No2001-E-01-2)

摘　　要：目的研究人尿萃取物CDA-Ⅱ(cell d ifferentiation a-gent-Ⅱ,又名尿多酸肽)对人胶质瘤细胞SWO-38抑制增殖及诱导分化作用。方法应用MTT法、集落形成试验检测CDA-Ⅱ对SWO-38细胞增殖的影响;通过光镜观察和免疫组化鉴定细胞分化;利用裸鼠抑瘤实验观察CDA-Ⅱ抗胶质瘤生长的作用。结果一定浓度的CDA-Ⅱ可使SWO-38细胞增殖与集落形成均明显受抑,并呈剂量依赖性。经1 g.L-1CDA-Ⅱ处理后,光镜观察发现SWO-38细胞胞体变大,核浆比减小,突起增多,表现出向成熟的星形细胞分化现象。免疫组化显示胶质纤维酸性蛋白(glial fibrillary ac id ic prote in,GFAP)表达增强。体内抑瘤试验见高、低剂量CDA-Ⅱ均可抑制裸鼠移植瘤生长,抑瘤率为79.94%、42.77%(P<0.05,n=10)。结论CDA-Ⅱ不仅能够抑制人胶质瘤SWO-38细胞的生长,而且能诱导SWO-38细胞分化,是一种很有应用前景的诱导分化剂。Aim To investigate the effect of CDA-Ⅱ （Uroacitide） which is a healthy human urine extraction on the proliferation inhibition and cellular differentiation of human glioma cells SWO-38. Methods Inhibition of SWO-38 cell proliferation was determined using MTT assay and colony formation assay. Cellular differentiation was determined with light microscopy observation and immunohistochemistry method. Nude mice were injected sc with cells SWO-38, and after tumor growing daily ip with CDA-Ⅱ for 28 days, observe the inhibitory effect. Results After SWO-38 cells treated with various concentration of CDA-Ⅱ, cell proliferation and colony formation were significantly inhibited in a dose-dependent manner. Light microscopy revealed that CDA-Ⅱ could induce SWO-38 cells differentiate toward astrocyte with increased cytoplasm and cytoplasmic processes and decreased nucleus/cyto- plasm rate. Furthermore, SWO-38 cells expressed more GFAP protein. CDA-Ⅱ at dosage of 0. 5g·kg^-1· d^-1 and 2 g·kg^-1·d^-1 with ip administration for 4 weeks significantly suppressed the growth of human glioma cells SWO-38 in nude mice, the inhibition rates were 42.77% and 79. 94% , respectively （P 〈0. 05 ,n = 10 ）. Conclusion CDA-Ⅱ not only inhibited growth of human glioma cells SWO-38, but also induced differentiation. It was a potential induce-differentiation reagent.

关 键 词：CDA-11 人胶质瘤SWO-38细胞 增殖 诱导分化 

分 类 号：R-332[医药卫生] R329.25