环氧合酶-2表达上调与人肝细胞癌新生血管生成的关系  

The correlation between up-regulation of cyclooxygenase-2 expression and hepatocellular carcinoma angiogenesis

作　　者：左朝晖[1] 谌忠友[1] 肖斌生[1] 周晓[1] 罗建红[1] 张理[1] 康忠诚[1] 

机构地区：[1]湖南省肿瘤医院普通外科,湖南长沙410013

出　　处：《中国普通外科杂志》2006年第1期40-44,共5页China Journal of General Surgery

基　　金：湖南省卫生厅科研基金资助项目(B2003122)

摘　　要：目的探讨环氧合酶-2(COX-2)表达上调与人肝细胞癌(HCC)新生血管生成的关系。方法采用免疫组化法和逆转录-聚合酶链反应法检测8 0例肝癌组织中COX-2,VEGF,bFGF,Ang-2和CD 3 4的表达情况;用W eidner分析法计算CD 3 4标记的平均微血管密度(MVD)。结果COX-2,VEGF,bFGF和Ang-2在HCC中表达率分别为7 5.0%,6 2.5 0%,6 0.0%和6 1.2 5%。VEGF,bFGF和Ang-2在COX-2强阳性组的免疫染色积分分别是5.9 8±1.1 6,4.5 7±0.2 6和5.8 7±0.1 2;而在COX-2中弱阳性组的积分分别是3.3 0±0.2 2,2.6 1±0.1 6和2.6 3±0.1 3;VEGF,bFGF和Ang-2在COX-2强阳性组的表达率分别是1 0 0.0%(9 5/9 5),9 4.2 9%(3 3/3 5)和9 7.1 4%(3 4/3 5),而在中弱阳性组分别是6 0.0%(1 5/2 5),6 0.0%(1 5/2 5)和6 0.0%(1 5/2 5)。两组的新生血管生成差异有显著性(P<0.0 1);COX-2与VEGF,bFGF和Ang-2表达呈明显正相关性(r分别为0.8 5 7,0.706,0.9 0 1;P分别<0.0 1)。MVD在COX-2强阳性组和中弱阳性组分别为7 3.1 8±1 2.4 3和33.42±7.52(P<0.0 1),COX-2与MVD呈正相关(r=0.8 1 2,P<0.0 1)。结论COX-2高表达与HCC血管生成密切相关。Objective To explore the correlation between up-regulation of eyclooxygenase-2 （COX-2） expression and hepatocellular carcinoma （ HCC ） angiogenesis. Methods The expression of COX-2 , vascular endothelial growth factor （ VEGF ） , basic fibroblast growth factor （ bFGF ） and angiopoientin-2 （ Ang-2 ） were examined in eighty matched sets of HCC specimens using immunohistochemistry and reverse transcription- polymerase chain reaction （ RT-PCR ） . Results In HCC, the expression rate of COX-2 , VEGF, bFGF and Ang-2 was 75. 0%, 62. 5%, 60. 0% and 61. 25%, respectively. lmmunohistochemical staining scores of VEGF, bFGF and Ang-2 were 5. 98 ± 1. 16, 4. 57 ± 0. 26 and 5.87 ±0. 12, respectively in strongly postive group of COX-2; and were 3.30 ±0.22,2.61 ±0. 16 and 2.63 ± 0. 13, respectively in moderately weak postive group of COX-2. The expression rates of VEGF, bFGF and Ang-2 were 100.0% （95/95） ,94.29% （33/35） and 97. 14% （34/35） , respectively in strongly postive group of COX-2; and were 60. 0% （15/25） ,60. 0% （15/25） and 60. 0% （ 15/ 25 ）, respectively in moderately weak postive group of COX-2. There was significant difference in HCC angiogenesis between the two groups （ P 〈 0.01 ） , and COX-2 expression was significantly correlated with VEGF,bFGF and Ang-2（ r =0. 857,0.706,0.901 ,respectively and all P 〈0. 01 ）. MVD was 73. 1.8±12.43 and 33.42 ± 7. 52 in strongly postivc group of COX-2 and moderately weak postive group of COX-2, respectively（ P 〈0.01） ,and COX -2 was significantly correlated with HCC MVD（ r = 0. 812, P 〈0.01 ）. Conclusions Up-regulation of COX-2 expression correlates with tumor angiogenesis in HCC.

关 键 词：癌 肝细胞 肝肿瘤 环氧合酶-2 

分 类 号：R730.261[医药卫生—肿瘤] R735.7[医药卫生—临床医学]