藤黄菌素和山萘黄素对人白血病细胞系HL-60体外增殖的抑制作用  

Inhibitory effect of luteolin and kaempferol on proliferation of human leukemic cell line HL-60 in vitro

作　　者：林小聪[1] 刘新光[1] 陈伟珠[1] 梁念慈[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023

出　　处：《实用肿瘤杂志》2006年第1期31-35,共5页Journal of Practical Oncology

基　　金：教育部科学技术研究重点项目(No.03096);广东省科技计划项目(No.2002C30109);湛江市科技局科技攻关计划项目(No.020101)

摘　　要：目的观察藤黄菌素和山萘黄素对HL-60细胞体外增殖的抑制作用及其对细胞周期的影响。方法分别采用台盼蓝拒染法及流式细胞术,在不同条件下测定细胞增殖的抑制率及细胞周期的分布。结果应用台盼蓝拒染法,藤黄菌素和山萘黄素能显著抑制HL-60细胞的体外增殖并且呈现时效及量效关系。流式细胞术的结果表明,藤黄菌素和山萘黄素作用2小时可使HL-60细胞周期分别阻滞于G2/M期和G0/G1期。结论藤黄菌素和山萘黄素是两种能够明显抑制HL-60细胞体外增殖,影响其细胞周期分布的黄酮类化合物。Objective To study the influence of luteolin and kaempferol on proliferation and cell cycle of human leukemic cell line HL-60 in vitro . Methods The cell growth was tested by trypan blue stain assay and the cell cycle was analysed by flow cytometry in the various conditions. Results Using the trypan blue stain assay it was found that luteolin and kaempferol inhibited the proliferation of HL-60 cell strongly,the higher dose and/or the longer time,the more obvious effects. Flow cytometry analysis showed that luteolin and kaempferol could arrest cell cycle progression in G2/M and G0/G1 phase in two hours,respectively. Conclusion Luteolin and kaempferol are effective flavonoid compounds which can inhibit the proliferation and affect the cell cycle of HL-60 cell in vitro.

关 键 词：白血病 藤黄菌素 山萘黄素 细胞系 细胞周期 

分 类 号：R733.7[医药卫生—肿瘤] R73-36[医药卫生—临床医学]