槲皮素在体外对NB4细胞形态及VEGF分泌的影响  

Effects of quercetin on morphology and VEGF secretion of leukemia cells NB4 in vitro

作　　者：钟璐[1] 陈芳源[1] 王海嵘[1] 滕晔[1] 王晨[1] 欧阳仁荣[1] 

机构地区：[1]上海第二医科大学附属仁济医院血液科,200001

出　　处：《中华肿瘤杂志》2006年第1期25-27,共3页Chinese Journal of Oncology

摘　　要：目的探讨槲皮素对NB4细胞的形态、血管内皮生长因子(VEGF)表达的影响。方法以NB4细胞为研究对象,采用Wright染色法观察细胞形态学;AnnecxinⅤ标记检测细胞凋亡率;采用ELISA法检测VEGF含量水平。结果(1)经槲皮素处理后,细胞形态学上出现凋亡特征性改变;(2)槲皮素处理后,NB4细胞凋亡率明显增加;(3)槲皮素处理后,NB4细胞显著降低VEGF的分泌水平。结论槲皮素在体外能抑制白血病细胞NB4生长并诱导其凋亡;槲皮素可抑制白血病细胞分泌VEGF。Objective To investigate the effects of quercetin on cell morphology and VEGF expression of acute myeloblastic leukemia cells NB4 in vitro. Methods The cytomorphology of NB4 cells was assessed by Wright-stain, apoptosis rate by apoptotic marker Annexin V, and VEGF secretion level by ELISA. Results Typical apoptosis was found in NB4 cells after treatment with quercetin. Apoptotic marker Annexin V analysis showed that the apoptotic rate of NB4 cells was increased after treatment with quercetin. The secretion of VEGF of NB4 cells was significantly decreased after treatment with quercetin. Conclusion Quercetin can induce apoptosis and inhibit secretion of VEGF in NB4 leukemia cells.

关 键 词：槲皮素 白血病细胞NB4 血管内皮生长因子 细胞形态 VEGF分泌 

分 类 号：R733.7[医药卫生—肿瘤]