氧环境对血管内皮生长因子、色素上皮衍生因子的影响及其与血管发育的关系  

Effect of oxygen on vascular endothelial growth factor,pigment epithelium derived factor and its relation with retinal vascularization

作　　者：俞亦龄[1] 刘坤[1] 万瑾[1] 郑华[1] 肖红蕾[1] 佘振珏[1] 周国民[1] 

机构地区：[1]复旦大学上海医学院解剖与组织胚胎学系,上海200032

出　　处：《解剖学杂志》2006年第1期11-13,F0002,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30270699)教育部重大重点项目(102096)上海市曙光学者计划

摘　　要：目的：研究视网膜发育过程中血管内皮生长因子(VEGF)和色素上皮衍生因子(PEDF)所起的调节作用及其机制。方法：C57BL/6J 小鼠从出生后7～12 d 饲养于75%氧环境,分别用抗 VEGF、抗 PEDF 和抗 CD31抗体作免疫组织化学标记视网膜切片。结果：持续处于高氧环境时,VEGF 表达处于低水平而 PEDF 快速上升,血管生长减缓。高氧处理5 d 后回到普通环境,VEGF 的表达迅速上升而 PEDF 却逐渐减少,出现了一过性的血管快速增长现象。结论：氧含量变化可调节 VEGF 和 PEDF 表达,参与视网膜血管发育。Objective：To determine the role of vascular endothelial growth factor （VEGF） and pigment epithelium derived factor （PEDF） in vascularization of the developing retina. Methods： Retinas were harvested at different postnatal days from C57BL/6J mice that have been maintained at ambient oxygen or exposed to 75% oxygen from P7 to P12. Immunostaining of VEGF, PEDF and CD31 was performed. Results：When the mice were exposed to 75% oxygen, the expression of VEGF was inhibited and expression of PEDF greatly enhanced; vascularization was slowed down. When exposed to the normal air condition 5 days later, the expression of VEGF increased sharply and peaked at P17. Conclusion： Oxygen can regulate the expression of VEGF and PEDF, and is involved in vascular development in retina.

关 键 词：血管内皮生长因子 色素上皮衍生因子 视网膜 血管发育 

分 类 号：R774.1[医药卫生—眼科]