血清p16基因甲基化联合CYFRA21-1检测在肺癌诊断中的应用  

Significance of Detection of p16 Gene Methylation and CYFRA21-1 in Serum for Diagnosis of Lung Cancer

作　　者：姚群峰[1] 宁勇[1] 张利平[2] 周宜开[2] 

机构地区：[1]湖北中医学院医学检验与技术系,邮政编码武汉430060 [2]华中科技大学同济医学院公共卫生学院

出　　处：《微循环学杂志》2006年第1期29-31,共3页Chinese Journal of Microcirculation

基　　金：湖北省教育厅优秀中青年项目(Q200516001)

摘　　要：目的:通过联合检测血清DNA中p16基因的异常甲基化以及血清细胞角蛋白CYFRA21-1的含量,以探讨两者联合检测在肺癌早期诊断中的临床意义。方法:分离提取53例肺癌患者的外周血血清DNA,采用巢式甲基化特异性PCR方法检测p16基因的甲基化状态,同时应用放射免疫法测定血清CYFRA21-1的含量,并与18例肺部良性疾病患者(BLD)和25例健康体检者作对照,分析两者联合应用的临床诊断价值。结果:在38例肺癌患者血清中检测到了p16基因的甲基化,阳性率为71.7%,而CYFRA21-1的阳性率为64.2%;平行联合检测时敏感性增至89.4%。BLD组中p16基因甲基化与CYFRA21-1阳性率分别为22.2%和11.1%;健康对照者血清中p16基因均无异常甲基化。结论:联合检测血清p16基因甲基化与CYFRA21-1含量可提高肺癌诊断的敏感性和特异性,对于肺癌的早期诊断具有重要意义。Objective: To evaluate the feasibility of combined detection of p16 gene methylation and CYFRA21-1 in serum as an approach for diagnosis of lung cancer.Method: Serum DNA was extracted from 53 patients with lung cancer. The methylation of p16 gene was detected by nested methylation specific polymerase chain reaction.Serum content of CYFRA21-1 was determined by radioimmunoassay.In addition,the results from 18 patients with benign lung disease and 25 healthy individuals were also analyzed as control.Results: Aberrant promoter methylation of the p16 gene was detected in 38 of 53 (71.7%) of the serum samples.The positive rates of CYFRA21-1 were 64.2%(34/53).Parallel combined test increased the diagnostic sensitivity to 89.8%.The positive rates of p16 gene methylation and CYFRA21-1 of the group BLD were 22.2% and 11.1%,respectively. No aberrant rmethylation of the p16 gene was found in 25 healthy individuals.Conclusion: Combined detection of p16 gene methylation and CYFRA21-1 in serum could increase the sensitivity and specificity in diagnosing lung cancer.

关 键 词：肺癌诊断 CYFRA21-1 P16基因甲基化 检测 原发性支气管肺癌 特异性标志物 早期诊断率 联合 血清 痰细胞学检查 

分 类 号：R734.2[医药卫生—肿瘤] R735.2[医药卫生—临床医学]