凝血栓蛋白1基因异常甲基化与大肠腺癌关联  

Aberrant Methylation of Promoter CpG Islands for Thrombospondin 1 Gene in Colorectal Adenocarcinoma

作　　者：张友才[1] 陈永平[1] 李骥[1] 王晓东[1] 邓长生[2] 朱尤庆[2] 龚玲[1] 

机构地区：[1]温州医学院附属第一医院,325005 [2]武汉大学中南医院,435005

出　　处：《实用癌症杂志》2005年第6期588-590,592,共4页The Practical Journal of Cancer

基　　金：湖北省科技攻关计划课题(编号2002AA301C84)

摘　　要：目的探讨凝血栓蛋白1(THBS1)基因启动子CpG岛异常甲基化与大肠腺癌及其临床病理特征的关联。方法THBS1基因甲基化状态用甲基化特异性PCR检测。结果大肠腺癌、癌旁组织中,THBS1基因启动子CpG岛甲基化率的差异有显著性(χ2=5.93,P=0.025);老年患者肿瘤组织中THBS1基因甲基化率明显高于非老年患者(χ2=5.68,P=0.017),直径≥3cm的肿瘤组织中THBS1基因甲基化率显著高于直径<3 cm的肿瘤(χ2=4.16,P=0.041),C期和D期肿瘤组织中THBS1基因甲基化率显著高于A期或B期肿瘤(χ2=8.04,υ=2,P=0.018)。结论THBS1基因甲基化与大肠腺癌的发生有关,肿瘤以老年、晚期和直径较大的肿瘤多见。Objective To study the relationship between aberrant methylation of promoter CpG islands for thrombospondin （THBS） 1 gene and colorectal adenocarcinoma （CRAC） and its clinicopathological features. Methods Using Methylation-Special PCR （MSP） ,we studied the methylation status of promoter CpG islands for THBS1. Results The differences in the ratio of methylation of promoter CpG island for THBS1 gene between the tissue in CRAC and the controls was statistics signifieanees （X^2 = 5.93, P = 0.025）. There were all significant differences in the ratio of promoter CpG island for THBS1 gene between the elder CRAC and the non-elder CRAC （X^2 = 5.68, P = 0. 017）, between the tumor with lager tumor size （≥3 cm in diameter） and the small tumor size （X2 = 4.16, P = 0. 041）,between the tumor in Ducks C and D stages and Ducks A or B stages （X^2= 8.04,v = 2, P = 0. 018）. Conclusion The aberrant methylation of promoter CpG islands for THBS1 gene is associated with the carcinogenesis of colorectal adenocarcinoma, especially the elder patient ,and most tumors are in advanced stage and large tumor size.

关 键 词：大肠癌 凝血栓蛋白1基因 CPG岛 甲基化 

分 类 号：R735.34[医药卫生—肿瘤]