^(32)P胶体组织间注射抑制血管细胞增殖作用的研究  

The inhibition of ^(32)P irradiation on proliferation of smooth muscle cells and endothelial cells in rabbit tissue

作　　者：杨继文[1] 赵劼[1] 孟祥琴[1] 王乐[1] 马庆杰[1] 

机构地区：[1]吉林大学中日联谊医院核医学科,吉林长春130033

出　　处：《吉林医学》2006年第2期119-120,共2页Jilin Medical Journal

摘　　要：目的:观察32Pβ电离辐射对血管平滑肌和血管内皮细胞增殖和凋亡的影响。方法:不同放射浓度(0,0.37,3.7,37,74kBq/2ml)32P照射血管平滑肌细胞和血管内皮细胞,用台芬蓝染色法,3H-TdR掺入法,流式细胞术和JMA法观察血管细胞活力、细胞增殖、细胞周期及细胞凋亡的变化。沿兔耳缘静脉将不同放射浓度(0,0.37,3.7,37,74kBq/2ml)的胶体32P均匀注入静脉周围,30d后取样观察血管细胞病理形态学改变,免疫组织化学技术检测增殖细胞核抗原和平滑肌肌动蛋白。结果:放射性浓度为0.37￣37kBq/2ml时血管细胞活力没有明显下降(P>0.05)。辐射对血管细胞增殖的抑制呈剂量依赖关系。受照射的血管细胞大部分停留在G0/G1期。放射性浓度>74kBq/2ml,细胞凋亡率明显上升,DNA裂解百分率显著升高(P<0.05),细胞结构呈凋亡样改变。病理形态学显示照射后组织血管数量减少。血管管腔闭塞。结论:32P胶体通过抑制海绵状血管瘤血管细胞增殖,促进血管细胞凋亡,最终导致血管闭塞来达到治愈海绵状血管瘤。Objective To study the effects of ^32P on apoptosis and proliferation of cultured smooth muscle cells （SMC） and endothelial cells （EC）, and to explore the mechanism of haemangioma treated by ^32P. Method The SMC and EC cultured in vitro were irradiated by ^32P with different closes. The trypan blue exclusion test, flow cytometry, JMA test, transmission electron microscopy and immunocytochemistry assay were used to investigate the effects of ^32P βray on apoptosis of SMC and EC, such as cell viability, cell apoptosis rate, DNA fragmentation, cell ultrastructural changes and related gene expression. Results There were no significant changes of SMC and EC viability, cell apoptosis rate, DNA fragmentation and cellular ultrastructure in low dose （〈 3.7kBq/2ml） irradiation group, compared with control group. High-dose （≥3.7 kBq/2ml） radiation on SMC and EC showed that viable cell proportion was markedly decreased, while cell apoptosis rate and DNA fragmentation were significantly increased, and cell ultrastructure was destroyed. SMC and EC occurred. Conclusion Low-dose ^32P β- radiation could inhibit completely SMC and EC proliferation and high-dose could result in significant SMC and EC apoptosis.

关 键 词：组织间注射治疗 海绵状血管瘤 ^32P 

分 类 号：R739.5[医药卫生—肿瘤]