补肾醒脑方对实验性血管性痴呆大鼠脑神经细胞Fas、Bcl-2基因表达的影响  

Effects on Expression of Fas or Bcl-2 Cerebral Neurocyte Apoptosis Gene of Experimental Vascular Dementia Rats with Bushen Xingnao Prescription

作　　者：郑攀[1] 封银曼[1] 郑宏[2] 夏寒星[1] 姚建平[1] 

机构地区：[1]河南中医学院,河南郑州450008 [2]河南中医学院第一附属医院,河南郑州450000

出　　处：《河南中医》2006年第2期25-27,共3页Henan Traditional Chinese Medicine

基　　金：2001年河南省杰出青年科学基金项目(编号:0112001300)

摘　　要：目的:探讨血管性痴呆(VD)病理机并研究补肾醒脑方对血管性痴呆大鼠脑神经细胞Fas、Bcl-2基因表达的影响。方法:采用反复夹闭双侧颈总动脉伴低血压造成拟血管性痴呆大鼠模型,通过免疫组化法检测在脑缺血再灌注后不同时间蛋白表达的变化,采用病理图文分析系统,测定Fas、Bcl-2染色阳性细胞的灰度值。结果:补肾醒脑高、低剂量组可抑制促凋亡基因Fas蛋白的表达,诱导抑凋亡基因Bcl-2蛋白的表达。结论:补肾醒脑方能抑制脑神经细胞凋亡,调节凋亡基因的表达,缓解兴奋性毒性损伤,保护大脑神经细胞,这可能是补肾醒脑方治疗VD的作用机制之一。Objective：To study the pathology mechanism of vascular dementia （VD）rats and study bushen Xingnao（BSXN）Prescription＇s effects on expression of Fas or Bcl-2 cerebral neurocyte apoptosis gene.Methods：The experimental model rats with VD were made by clipping two sides of total artery in the neck repeatedly together with hypotension.The immunohioto chmical method was used to observe the change of protein expression after length of cerebral ischemia reperfusion.The pathological picture analyze system was used to determine the tonal value of Fas,Bcl-2 positive cells.Results：Both the high and low dose BSXN can inhibit the excessive expression of urg-apoptosis Fas gene and induce the expression of anti-apoptosis Bcl-2 gene after corebral ischernia reperfusion.Conclusion：BSXN Prescription can inhibit neurocyte apoptosis,regulationg the expression of promoting/resisting apoptosis gene,abationg excitatory toxic infury,protecting cerebral neurecyte,which may be cne of the mechanisms of Bushen Xingnao Preseription＇s treatment for VD.

关 键 词：补肾醒脑方 血管性痴呆 细胞凋亡 FAS BEL-2 基因表达 

分 类 号：R749.16[医药卫生—神经病学与精神病学]