聚-L-赖氨酸的合成与表征  

Synthesis and Characterization of Poly(L-Lysine)

作　　者：胡海梅[1] 潘仕荣[1] 温玉婷[1] 郭振寰[1] 

机构地区：[1]中山大学附属第一医院,广东广州510080

出　　处：《中山大学学报（自然科学版）》2006年第1期64-68,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省科技攻关基金资助项目(2KB02902J)

摘　　要：以L-赖氨酸为原料,先制备NΕ-苄氧羰基-L-赖氨酸,光气法合成NΕ-苄氧羰基-L-赖氨酸-N-羧酸酐,并以三乙胺为引发剂,在氯仿中引发聚合,生成聚(NΕ-苄氧羰基赖氨酸),经无水溴化氢脱苄得到聚-L-赖氨酸。其聚合度由黏度法测定,化学结构由IR1、H NMR证实,聚(NΕ-苄氧羰基赖氨酸)和聚-L-赖氨酸分子均以Α-螺旋构型存在。由本法制备的聚-L-赖氨酸可获很好的产率(>70%),其相对分子质量由单体/引发剂摩尔比控制。ε-Z-lys was prepared by a reaction of L-Lysine with carbobenzoyloxy chloride, Lys （Z）-NCA was synthesized by a reaction of ε-Z-lys with triphosgene in THF. Poly-L-lysine （Z） [ PLL （ Z ） ] was polymerized by initiation of ethylamine （TEA） in chloroform. Poly-L-lysine （PLL） was obtained by a deprotecting reaction with anhydrous hydrogen bromide. The polymerization degree was measured by viscosimetry. The chemical structure of PLL was characterized by IR and ^1H NMR. The results demonstrated that PLL can be prepared by this procedure with good yied （more than 70% ） and its molecular weight can be controlled by adjusting the molar ratio of monomer to initiator. It was showed that PLL （Z） and PLL molecules existed in a-helix polypeptide in films.

关 键 词：L-赖氨酸 N^E-苄氧羰基-L-赖氨酸 聚(N^E-苄氧羰基赖氨酸) 聚-L-赖氨酸 三乙胺 

分 类 号：O623.735[理学—有机化学]