散发性阿尔茨海默病与ApoE及LRP基因3号外显子C/T多态性的关联分析  

Correlation of the polymorphisms of apolipoprotein E gene and low-density lipoprotein receptor-related protein gene with sporadic Alzheimer’s disease

作　　者：冯亚青[1] 王建华[1] 赵大卫[1] 国雪[1] 孙占用[1] 哈志远[1] 

机构地区：[1]河北省人民医院老年神经科,河北省石家庄市050051

出　　处：《国际神经病学神经外科学杂志》2006年第1期9-12,共4页Journal of International Neurology and Neurosurgery

基　　金：河北省卫生厅资助项目(200001053)

摘　　要：目的探讨载脂蛋白E(ApoE)基因与低密度脂蛋白受体相关蛋白(LRP)基因3号外显子C/T多态性在散发性阿尔茨海默病(SAD)发病中的作用。方法应用聚合酶链反应和限制性片段长度多态性方法,检测了56例SAD患者和75例正常老年人的ApoE基因多态性和LRP基因3号外显子C/T多态性,并对SAD与ApoE和LRP各基因型及等位基因进行了关联分析。结果与对照组比较,SAD患者等位基因ε4频率显著升高(x2=11.36,P<0.01),SAD患者与等位基因ε4均呈正关联(OR=3.29,CI1.36～7.95)。SAD患者与LRP等位基因C呈正关联(OR=1.83,P<0.05),与等位基因T呈负关联(OR=0.55,P<0.05)。结论ApoE等位基因ε4和LRP等位基因C可能是SAD发病的危险因素,ApoE和LRP基因多态性与SAD之间有密切相关性。Objective To explore the roles of the polymorphisms of apolipoprotein E （ApoE） gene and exon3 polymorphism of lowdensity lipoprotein receptor-related protein （LRP） gene in the development of sporadic Alzheimer＇ s disease （SAD）. Methods The polymorphisms of ApoE gene and exon3 polymorphism of LRP gene were detected by the polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） technique in 56 patients with SAD and 100 normal controls. Results The frequency of allele ε-4 in patients with SAD increased significantly compared with that of the normal controls （x^2 = 11.36, P 〈 0.01 ）. SAD was positively corre- lated with allele e4 （ OR = 3.29, CI 1.36-7.95 ） and LRP allele C （ OR = 1.83, P 〈 0.05 ）, but was negatively associated with LRP allele T （ OR = 0.55, P 〈 0.05 ）. Conclusions The allele ε-4 of ApoE gene and allele C of LRP gene might be the risk factors for SAD. There may be a a genetic association between the polymorphisms of ApoE gene and LRP gene and SAD.

关 键 词：阿尔茨海默病 载脂蛋白E基因 低密度脂蛋白受体相关蛋白基因 多态性 

分 类 号：R749.16[医药卫生—神经病学与精神病学]