肿瘤患儿外周血单个核细胞采集及其对受体造血恢复的影响  

Peripheral Blood Stem Cell Collection in Pediatric Donors with Malignancies and Its Significance on Hemato-poietic Recovery of Recipients

作　　者：罗长缨[1] 陈静[1] 潘慈[1] 周敏[1] 宋德莲[1] 蒋黎敏[1] 薛惠良[1] 汤静燕[1] 顾龙君[1] 

机构地区：[1]上海交通大学医学院附属新华医院上海儿童医学中心血液肿瘤科,上海200127

出　　处：《实用儿科临床杂志》2006年第3期148-150,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的回顾分析26例白血病和实体瘤患儿外周血干细胞采集效果及其对受体造血恢复影响,筛选可能与采集效率提高和受体造血恢复相关的因素。方法总结26例供体的49次粒系集落刺激因子(G-CSF)动员后的外周血采集过程,统计分析供体动员剂量、采集时外周血白细胞、收获的单个核细胞(MNC)和CD34+细胞数、受体造血恢复三者间的相关性。结果26例供体均能很好耐受动员和采集过程。采集物MNC和CD34+细胞中位数分别为4.5×108/kg和1.9×106/kg。动员剂量与采集物中MNC数呈正相关,与采集物CD34+细胞数无相关。采集当天供体外周血白细胞总数对采集细胞量无影响,而MNC水平与CD34+细胞采量正相关。结论受体的造血恢复与采集物中的CD34+细胞数可能存在相关性。因此这类患儿的采集时有必要动态监测其动员时的外周血CD34+细胞水平,确定最佳采集时间,以获取足够的CD34+细胞采量。Objective Data on the leukapheresis fronl 26 pediatric patients with hematologic or ,solid malignancies was retrospectively evaluated to screen predictive factors affecting the efficacy of peripheral blood stem celI（PBSC） collection from donors, as well as hematopoietic recovery in recipients. Methods We present our experience with 49 apberesis from 26 granuloeyte - colory Stimulating factor mobilized donors and analyzed the correlations between the mobilization, the leukocyte count in the donor peripheral blood and the MNC and CD34^＋ cell yields in collecting products and the neutrophil and platelet recovery of recipients. Results The process of mobilization and apheresis were well tolerated by our pediatric donors. The median numbers for harvested MNCs and CD34^＋ cells were 4.5 × 10^3/kg and, 1.9 ×10^6/kg of recipient body weight, respectively. Mobilizing dose pisitively affected the number of mononuclear ceus （MNC） but not CD^34 ＋ cells in the apberasis products. The CD34^＋ cell number in the apheresis product was influenced significantly by donor circulating MNC on the day of harvest and correlated with recipient＇s engraftment after PBSC was reinfused. Conclusions The MNC yield was stable and met with the demand for autologous stem cell transplantation while the CD34^＋ cell number varies obviously from each donor, Since a rapid engraftment was asssociated with a high number of CD34^＋ cells collected, which was in turm predicted by the level of the pre - apheresis CD^34 ＋ cells in the peripheral blood of donors, it is necessary,sary to monitor the donors CD34^＋ cell during mobilization to determine the optinmal time for apheresis.

关 键 词：造血干细胞采集 外周血 造血恢复 儿童 肿瘤 

分 类 号：R730.5[医药卫生—肿瘤]