TRIO扩增和高表达在膀胱癌的进展和癌细胞快速生长中的作用  

TRIO amplification and overexpression is associated with tumor development in bladder cancer

作　　者：郑敏[1] Moch H 

机构地区：[1]华南肿瘤学国家重点实验室 中山大学肿瘤防治中心妇科,广州510060 [2]瑞士巴塞尔大学病理所

出　　处：《实用肿瘤学杂志》2006年第1期1-6,共6页Practical Oncology Journal

基　　金：瑞士国家自然科学基金资助(编号:E420663)

摘　　要：目的比较基因杂交（CGH）研究发现染色体5p扩增与膀胱癌的进展有关；TRIO基因位于扩增区，其编码的蛋白在细胞周期调节中起主导作用。本研究在于了解TRIO的扩增和表达是否与膀胱癌的发展有关。方法用原位杂交法（FISH）分析位于5p15．31～5p15．1上的7个基因（TAS2R1，ADCY2，DNAH5，CTNND2，TRIO，ANKH和MYO10）在膀胱癌组织微阵列的作用，其中扩增率最高的是TRIO基因。含有2317例膀胱癌的大组织微阵列进一步调查TRIO的扩增情况；RNA原位杂交法和Northern Blot法进一步了解TRIO在膀胱癌组织的表达情况。结果显示TRIO扩增与肿瘤分期、分化程度和肿瘤细胞快速增殖密切相关。TRIO扩增仅见于7例／456例早期膀胱肿瘤（pTaG1／G2）（1．5％），而在膀胱癌（pT1—4）高达62例／485例（12．8％）。膀胱肿瘤组织微阵列RNA原位杂交法和Northern Blot法证实TRIO在膀胱癌组织高表达。结论TRIO扩增和高表达可能在膀胱癌的发展过程中发挥重要作用。Objective CGH exhibited that 5p amplification is associated to progression of bladder cancer. The TRIO gene maps to 5p 15.1 - p14 and encodes a protein with a putative role in cell cycle regulation. This study is designed to find out if the amplification and expression of TRIO is correlated to the progression of bladder cancer. Methods The fluorescence in situ hybridization （FISH） analyzed the influence of 7 genes, which located in 5p 15.31 - 5p 15.1 including TAS2R, ADCY2, DNAH5, CTNND2, TRIO, ANKH and MYO10, in bladder cancer tissue microarray TRIO showed higher rate of amplification. Bladder cancer tissue microarray including 2317 cases were further used to investigate amplification of TRIO. RNA in situ hybridization and Northern blot analysis were used in this study to investigate expressionof TRIO. Results The FISH study from a tissue microarray containing samples from 1636 bladder tumors exhibits that TRIO amplification was strongly associated with invasive tumor phenotype and high tumor grade （ P〈0.0001 respectively）. Only 1.5% （7 of 456） with pTaG1/G2 tumors but 12.8% （62 of 485） with pT1 -4 carcinomas had TRIO amplification. RNA in situ hybridizaion and Northern blot analysis confirmed that TRIO is overexpressed in amplified tumors. Conclusion TRIO is frequently amplified and overexpressed in invasive bladder cancer （stage pT1 - 4）. TRIO amplification/overexpression might have a potential role in bladder cancer progression.

关 键 词：膀胱癌 FISH RNA原位杂交 NORTHERN BLOT法 组织微阵列 

分 类 号：R737.14[医药卫生—肿瘤] R777.5[医药卫生—临床医学]