孕晚期羊水细胞的荧光原位杂交  

Interphase FISH of amniocytes in the third trimester

作　　者：游泽山[1] 黄珈[1] 陈建生[1] 陈宝江[1] 王彩玲[1] 

机构地区：[1]中山大学附属第一医院妇产科,广州510080

出　　处：《中华围产医学杂志》2006年第1期18-20,共3页Chinese Journal of Perinatal Medicine

基　　金：广东省计划生育委员会基金(200105)

摘　　要：目的前瞻性研究孕28周以后羊水细胞的荧光原位杂交。方法采用盲法,对孕 28周后需进行产前诊断的32例孕妇在取脐带血的同时抽吸5 ml羊水进行荧光原位杂交。结果 32例孕妇发现染色体数目异常6例(18三体3例,21三体2例,47XYY 1例)。且羊水细胞荧光原位杂交结果与脐带血染色体核型分析的结果一致,符合率达100％。结论孕28周后羊水细胞荧光原位杂交,对常见的染色体数目异常诊断准确性高,有一定的临床推广价值。Objective To study the interphase FISH of cells from aminotic fluid in the third trimester prospectively. Methods Amniotic fluid （5ml） was obtained in 32 pregnant women in the third trimester who underwent cordocentesis for karyotyping. FISH was performed on uncultured amniocytes and the results were compared with karyotypes. Results Abnormal aneuploid were identified in 6 cases among the 32 women. The FISH results＇were consistent with the karyotypes. Conclusions Interphase FISH in third trimester amniocentesis can provide an accurate clinical diagnosis on common aneupoidies,

关 键 词：羊膜腔穿刺术 妊娠末期 原位杂交 荧光 

分 类 号：R714[医药卫生—妇产科学]