荧光原位杂交技术在微生物群落结构研究中的应用  

Application of Fluorescent in Situ Hybridization (FISH) to Environmental Microbial Community Structure

作　　者：李华芝[1] 李秀艳[1] 徐亚同[1] 

机构地区：[1]华东师范大学资源与环境学院环境科学与技术系,上海200062

出　　处：《净水技术》2006年第1期16-20,共5页Water Purification Technology

基　　金：国家高技术研究发展863计划专项资助(2003AA601020)

摘　　要：该文综述了荧光原位杂交技术的发展、技术原理及其在环境微生物群落研究中的应用,还探讨了该技术在应用中存在的问题,并对其应用前景进行了展望。In this paper, basic principles and its evolution of FISH were introduced, then its applications in environmental microbial community structure studies were summarized, its problems and shortages we,e discussed in an examination of past, present and future applications, and perspectives were expected about this technology.

关 键 词：荧光原位杂交 16S RRNA 寡核苷酸探针 微生物群落结构 

分 类 号：X703.1[环境科学与工程—环境工程] X172