胚胎发育相关基因精子转录本在白血病细胞系中的表达及其调节  

Expression of distal (testis) promoter of EDAG in leukemia cell lines and its regulation during cell differentiation

作　　者：安莉莉[1] 马小彤[1] 石洋[1] 林永敏[1] 郑国光[1] 吴克复[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所实验血液学国家重点实验室,天津300020

出　　处：《白血病．淋巴瘤》2006年第1期1-3,9,共4页Journal of Leukemia & Lymphoma

基　　金：高等学校博士学科点专项科研基金(20020023015)

摘　　要：目的探讨胚胎发育相关基因(EDAG)在白血病细胞中的调控机制。方法用半定量RT-PCR方法检测白血病细胞系中EDAG精子转录本EDAG-t的表达;使用不同的分化诱导剂诱导K562和U937细胞分化,检测EDAG-t的表达改变情况。结果在白血病细胞系K562和U937中也可检测到EDAG-t的表达;使用不同的分化诱导剂诱导K562和U937细胞分化,发现EDAG精子转录本和造血转录本的表达都会随细胞分化而下调,但它们的下调步伐不一致,总的说来,EDAG-t的减弱较EDAG-h慢。结论EDAG精子转录本在白血病细胞中的异常表达值得进一步研究。Objective To explore the regulation mechanism of EDAG expression in leukemia cells. Methods We detected the pattern of EDAG-t mRNA expression via RT-PCR in a panel of leukemia cell lines. Then we further explored the regulation of EDAG-t and EDAG-h expression during the differentiation of K562 and U937 cells. Results EDAG-t （human HEMGN-t） isoform could also be detected in the leukemia cell lines K562 and U937. And both HEMGN-t and HEMGN-h could be down regulated during the differentiation of K562 and U937 cells but the kinetics were different. Conclusion The regulatory mecha- nisms for HEMGN-t in testis and leukemia cell lines deserve further investigation.

关 键 词：胚胎发育相关基因精予转录本 白血病细胞系 

分 类 号：R733.7[医药卫生—肿瘤]