白细胞介素6在肾癌和膀胱癌细胞系中的表达  

IL-6 expression in the renal and bladder carcinoma cell lines

作　　者：钟华[1] 顾方六[1] 俞莉章[1] 丁义[1] 刘漓波[1] 杨定成[1] 

机构地区：[1]北京医科大学第一医院,北京医科大学泌尿外科研究所,北京医科大学第一医院大肠癌实验室

出　　处：《中华泌尿外科杂志》1996年第1期17-19,共3页Chinese Journal of Urology

摘　　要：应用ＩＬ－６依赖株增殖反应测定法和Ｎｏｒｔｈｅｒｎ印迹法检测ＧＲＣ－１肾细胞癌细胞系和ＢＩＵ－８７膀胱移行细胞癌细胞系培养上清液中ＩＬ－６活性水平及ＩＬ－６ｍＲＮＡ的表达，发现这两种细胞均能表达ＩＬ－６ｍＲＮＡ并具有自发性分泌ＩＬ－６的能力。IL-6 has been considered to be related to tumor immunity and can be secreted by a variety of tumor cells in vitro.With the IL-6-dependent cell line proliferating response method and the Northern blot technique,IL-6 expression of GRC-1 and BIU-87 cell lines was studied with respect to biologic activity and mRNA.The results revealed that the cells of both the cell lines do express IL-6mRNA and are able to produce IL-6spontaneously.

关 键 词：肾肿瘤 膀胱肿瘤 细胞系 白细胞介素6 

分 类 号：R737.11[医药卫生—肿瘤] R737.14[医药卫生—临床医学]