膀胱癌转化生长因子α和P53基因表达及生物学意义  

Transforming growth factor αand P53 gene expression in bladder cancer

作　　者：郭德荣[1] 李鸣[1] 顾方六[1] 周爱儒[1] 蔡碧娟[1] 郭应禄[1] 

机构地区：[1]北京医科大学第一医院,北京医科大学泌尿外科研究所,北京医科大学生化教研室

出　　处：《中华泌尿外科杂志》1996年第2期67-69,共3页Chinese Journal of Urology

基　　金：国家自然科学基金

摘　　要：通过现代分子生物学方法，对膀胱癌实体肿瘤的ＴＧＦα和Ｐ５３表达及生物学意义进行了探讨。３４例膀胱癌中２９例ＴＧＦαｍＲＮＡ表达，表达率为８５．３％（２９／３４）；１３例癌旁对照组织中，６例无表达，６例低表达，１例高于自身肿瘤表达。肿瘤的表达量是癌旁正常对照的２～５倍，统计学分析，Ｐ＜０．０１。１４例膀胱癌４例（２８．５％）Ｐ５３高表达，２例低表达，２例正常和８例癌组织未见表达。膀胱癌ＴＧＦα基因的高表达可能是膀胱癌发生发展的早期信号，Ｐ５３基因的变异表达可能主要参与癌细胞的分化，它可能与膀胱癌恶性表行相关。Transforming growth factor α(TGFα)and P53 mRNA were detected by northern blot analysis in patients with bladde cancer.29 of 34(85.3%) bladder carcinoma expressed TGFαand its level has been 2￣5 fold that of normal bladder tissue(p<0.01).The TGFa level was however not relevant to the tumor grade and stage.6 of 14 tumors had P53 mRNA detected,4 with high level and the other 2 with low expression.5 of the 6 P53 positive tumors were of grade 2 or grade 3.7 of 13(54%)normal epithelium tissue adjacent to the tumor has also been TGFαpositive but were of low level except in one.The results denoted that elevated TGFαmRNA might signal an early event in carcinogenesis of bladder cancer while abnormal expression of P53 gene might indicate indirectly the gene mutation and was relevant to the malignancy of bladder tumors.

关 键 词：膀胱肿瘤 转化生长因子 P53基因 基因表达 

分 类 号：R737.14[医药卫生—肿瘤]