内皮脂酶在载脂蛋白E基因缺陷小鼠主动脉粥样斑块中的表达  

Affection of ApoE Gene - deficiency on the Expression of Endothelial Lipase in the Wall of Artery

作　　者：许灿新[1] 王春[2] 朱炳阳[1] 秦旭平[1] 廖端芳[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001 [2]南华大学护理学院,湖南衡阳421001

出　　处：《美国中华临床医学杂志》2006年第1期42-44,48,共4页American Journal of Chinese Clinical Medicine

摘　　要：目的内皮脂酶（endothelial lipase，EL）与动脉粥样硬化的发生、发展有密切关系。本研究探讨高脂喂养和apoE基因缺陷（apoE-，-）小鼠EL表达的差异。方法取apoE-/-小鼠作为研究对象，以普通饲料和高脂喂养正常小鼠为时照组，测定各组小鼠主动脉壁斑块的变化，免疫组化检测EL在主动脉的表达。结果正常喂养的apoE-/-小鼠的主动脉形成明显的斑块，高脂喂养小鼠没有形成斑块，但血管壁平滑肌细胞出现增殖；免疫组化检测显示普通饲料喂养的apoE-/-小鼠EL有明显表达，而高脂和普通饲料组喂养的正常小鼠没有表达。结论血管壁粥样斑块中EL的表达可能是促进apoE-/-小鼠动脉粥样斑块形成的主要原因。Objective Endothelial lipase plays an important role in the development of atherosclerosis（AS）.The object of study is to observe the expression of endothelial lipase in the apoE - deficient mice. Methods Male C57BL/6J mice and apoE - deficient mice were devided into apoE - deficient and eontrol group mice,the htters were further divided into 2 subgroups of common feedstuff and high- fat（（n = 10）. The serum total cholesterol, triglyeeride, high and low desity cholesterol were determined. The change of aortic wall and plaque lesion were observed, endothelial lipase was assayed by IHC Rerults ApoE - deficient mice devdoped plaque in the artery, but not in high - fat mice. The expression of endothelial lipase in aortic wall in the apoE - deficient mice was increased compared with those in the normal mice. Conclusion The expression of expression of endothelial lipase in aortic of apoE- deficient mice is the main cause of atherositie formation.

关 键 词：内皮脂酶 动脉粥样硬化 apoE基因缺陷 高脂喂养 载脂蛋白E 基因缺陷 小鼠 

分 类 号：R543.1[医药卫生—心血管疾病]