急性脑缺血再灌注中极化液的保护作用实验研究  

The Research on the Protection of GIK Effect on Acute Cerebral Ischemic Reperfusion

作　　者：张畅[1] 任艳丁[1] 刘爽[1] 

机构地区：[1]哈尔滨医科大学第四临床医学院,黑龙江哈尔滨150001

出　　处：《黑龙江医学》2006年第2期110-111,共2页Heilongjiang Medical Journal

摘　　要：目的观察极化液（GIK）对急性脑缺血的保护作用。方法实验于2004-06～2004-12于哈尔滨医科大学动物实验中心进行。取70只Wistar大鼠，随机分为10组，每组7只：随机分为：①正常组：不干预；②假手术组：仅分离MCA，不线拴；③单纯脑缺血30min：线拴法阻断MCA，脑缺血30min后断头处死：④脑缺血1h组：阻断MCA，脑缺血1h后断头处死；⑤再灌注2h组：阻断MCA，脑缺血1h后再灌注2h后处死；⑥再灌注5h组；⑦脑缺血30min＋GIK组：断头前30min给予普通胰岛素2U／kg腹部皮下注射，50％葡萄糖10g／kg，10％氯化钾0．25g/kg灌胃；⑧脑缺血1h＋GIK组；⑨再灌注2h＋GIK组；⑩再灌注5h＋GIK组：同前给药并造模，再灌注5h后处死。用高效液相色谱法测定急性脑缺血大鼠的脑缺血组织中谷氨酸（Glu）、天门冬氨酸（Asp）、硝酸还原酶法测定一氧化氮（NO）的含量。结果70只动物进入结果分析：①Glu含量：各缺血再灌注组显著高于假手术组，对照组应用GIK后可显著降低；②Asp含量：各缺血再灌注组显著高于假手术组，对照组，应用GIK后可显著低于缺血组；③NO浓度：缺血再灌注组显著高于假手术组，对照组。应用GIK后较缺血组有明显降低。结论GIK能减轻Glu、Asp、NO的神经毒性，起到对缺血脑组织的保护作用。Objective To observe the protection of GIK effect on acute cerebral ischemic.Methods The experiment was performed in the animal experimental center of harbin medical university from june 2004 to december 2004. totally 70 wistar rats were randomly divided into normal group, ischemic 30 rain group, ischemic 1 h group, ischemic 2 h group, reperfusion 2 h group, reperfusion 5 h group, isehemic 30 min ＋ GIK group, ischemic 1 h ＋ GIK group, ischemic 2 h ＋ GIK group, ischemic 5 h ＋ GIK group. Measure Glu and Asp with high performance liquid chromatogrophy, and nitric acid reduction enzyme law of NO. Result The content of Glu and Asp and NO is higher of ischemic group than of GIK group. Conclusion GIK can lighten the nerve toxicity of Glu, Asp and NO, and protect on the ischernic organisation.

关 键 词：神经病学 极化液 急性脑缺血 谷氨酸 天门冬氨酸 一氧化氮 

分 类 号：R743[医药卫生—神经病学与精神病学]