Twist基因表达与肝癌多耐药细胞侵袭的相关性研究  

TWIST AND THE METASTASIS OF MULTIDRUG RESISTANT CELLS OF HUMAN HEPATOCELLULAR CARCINOMA

作　　者：王其[1] 陈孝平[1] 关剑[1] 张万广[1] 张必翔[1] 

机构地区：[1]华中科技大学同济医院肝胆胰外科研究所,武汉430030

出　　处：《肝胆外科杂志》2006年第1期66-68,共3页Journal of Hepatobiliary Surgery

摘　　要：目的研究胚胎发育相关基因Twist在人肝癌多药耐药细胞系HepG2／ADM中的表达及意义。方法建立肝癌多药耐药细胞系HepG2／ADM,利用荧光定量PCR技术及免疫印记法分别检测Twist mRNA及Twist蛋白;多药耐药相关基因MDR 1mRNA在HepG2／ADM及其亲本细胞HepG2中表达的差异。结果Twist在HepG2／ADM中表达上调,与亲本细胞 HepG2相比,mRNA(9．34 vs 1,P<0．05),蛋白(10．64±0．56 vs1,P<0．05),具有显著性差异,MDR1mRNA表达在HepG2／ ADM中显著上调,是其亲本细胞的348．99倍(P<0．05)。结论 Twist在HepG2／ADM中表达上调,这可能使得HepG2／ ADM转移扩张能力增加。Objective To study the expression of Twist gene in HepG2 and its multidrug resistance sub-cell line Hepgl/ADM, Methods Using real time PCR to check the Twist mRNA and MDR1 mRNA levels in the muhidrug resistant cell line Hepg2/ADM and its mother cell line Hepg2 and western blotting to detect the twist protein in the two cell lines. Result Twist and MDR1 was up regulated in Hepg2/ADM cells. Conclusion Compared with its mother cells,Hepg2/ADM cells may have the more tendenee of metastasis owing to the up regulation of Twist gene.

关 键 词：TWIST 多药耐药 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]