尼膜同对脑出血大鼠血肿周围核转录因子-κB表达的影响  

Effects of nimotop on the expression of NF-κB in perihematomal brain regions of rats

作　　者：顾萍[1] 张勇[1] 丁新生[1] 高飞[1] 邓晓宣[1] 姚娟[1] 朱国庆[2] 

机构地区：[1]南京医科大学第一附属医院神经内科,江苏南京210029 [2]南京医科大学生理教研室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第3期153-155,188,F0002,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30170827)

摘　　要：目的:研究尼膜同对大鼠脑出血后核转录因子-κB(NF-κB)表达的影响。方法:将SD大鼠随机分成正常组、假手术组、脑出血模型组、尼膜同治疗组。采用VII型胶原酶注入到大鼠右侧苍白球制备脑出血模型,每组分别在术后6、24、48、96h4个时间点收取脑组织标本,免疫组化检测NF-κBP65蛋白表达,同时测量脑组织含水量。结果:与正常组和假手术组比较,大鼠脑出血后脑组织NF-κBP65表达以及脑组织含水量显著增高(P<0.01);与脑出血模型组比较,尼膜同治疗组NF-κBP65水平及脑组织含水量均显著降低(6h,P<0.05;24、48、96h,P<0.01)。结论:尼莫通可以抑制脑出血后NF-κB表达,这可能是尼膜同减轻脑出血后脑水肿的机制之一。Objective： To study the influence of nimotop on the expression of NF-κB in perihematomal brain regions of rats. Methods： Male Sprague -Dawley rats were randomly divided into normal group （group A） , pseudo-operation group （group B）, model group （group C） and nimotop treatment group （group D）.ICH models were induced in rats by injecting Ⅶ type collagenase into the fight globas pallidus. The brain tissue specimens in every group were respectively gathered at the time of 6 h,24 h ,48 h and 96 h after operation. The expression of NF-KB in rat brain after ICH was detected with immunohistochemistry method, at the same time the moisture in brain tissue was measured. Results： Compared with group A and group B , the expression of NF-κB and moisture in brain tissue in group C were obviously increased （P 〈 0. 01） . Compared with group C , the expression of NF-κB and moisture in brain tissue in group D were obviously decreased （P 〈 0.05 ,or P 〈 0.01 ）. Conclusion： Nimotop can reduce the expression of NF - κB in rat after ICH, which may be related with the anti-edema effect following ICH.

关 键 词：脑出血 核转录因子-ΚB 脑水肿 尼膜同 

分 类 号：R743.34[医药卫生—神经病学与精神病学]