电针“合谷”和“四白”穴对大鼠孤束核胃相关神经元电活动的影响  

Effect of Electroacupuncture of "Hegu"(LI 4) and "Sibai"(ST 2) on Electrical Activities of Stomach-related Neurons in the Nucleus of Solitary Tract of the Rat

作　　者：陈淑莉[1] 晋志高[1] 景向红[1] 张建梁[1] 蔡红[1] 逯波[1] 蒋瑾[1] 张璐[1] 刘爱红[2] 

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]美国南加州大学病理系

出　　处：《针刺研究》2006年第1期46-49,61,共5页Acupuncture Research

基　　金：国家自然科学基金资助项目(No.30472240);国家中医药管理局项目(No.2002LHR01;2000-J-P-49)

摘　　要：目的:用电生理学的方法探讨“合谷”穴与“四白”穴的传入信息在孤束核内的汇聚。方法:采用细胞外记录的方法,首先寻找到孤束核内对胃扩张敏感的神经元,然后分别电针刺激“合谷”穴和“四白”穴,观察其放电活动的变化。结果:共记录到278个有自发放电的孤束核神经元,以10 s为时间段,统计处理胃扩张前后各2 min的神经元放电数目,频率改变超出25%确定为胃扩张反应神经元,132个神经元被确定为胃扩张反应(敏感)神经元,146个没有明显反应。对132个胃扩张反应神经元做进一步的统计学分析,70个神经元的电活动被胃扩张刺激兴奋(70/132);62个神经元被胃扩张刺激抑制(62/132),其中36个神经元的放电活动被完全抑制。与胃扩张前(14.05±8.52 spikes/min)相比,兴奋性神经元的电活动增加(34.84±22.68 spikes/min)极为显著;抑制性神经元的放电变化也非常明显(由18.21±9.29 spikes/min变为7.30±6.20 spikes/min)。在132个胃扩张敏感神经元中,对电针“四白”穴和“合谷”穴均起反应的神经元有41个(41/132,占30.2%)。结论:电针“合谷”穴和“四白”穴和胃扩张可兴奋或抑制同一孤束核神经元的电活动,表明“合谷”穴和“四白”穴区的传入信息可汇聚到同一孤束核神经元上,提示孤束核很可能是合谷穴与口面部联系的中枢机制之一,从而为经络理论中的人体体表与体表上下的特定联系提供了新的实验证据。Objective： To study the effect of electroecupuncture （EA） of ＂Hegu＂ （LI 4） and ＂Sibai＂ （ST 2） on the electrical activities of stomach-related neurons in the nucleus of solitary tract （NTS） of the rat. Methods： Experiments were per, formed on 62 Wistar rats anesthetized with mixture solution of urethane （420 mg/kg） and chloralose （50 mg/kg, i. p. ）. The 4^th ventricle of the brain was exposed for inserting glass micropipettes （ 1μmdiameter, 10 - 30 mΩ in electrical resistance, and filled with 1 tool KCI＋2% pontamine sky blue）Into NTS under the microscopic observation to record electrical activities of neurons. After opening the abdominal cavity and making an incision at the junction between the pylorus and duodenum, a plastic balloon was inserted into the stomach for dilating it. EA （2-4 V, 20 Hz and duration of 2 min） was applied to the left ＂Hegu＂ （LI 4） and ＂Sibao＂（ST 2）. If the firing rate of the neuron decreased or increased by 25% or more after EA or gastric dilation stimulation, it was considered to be inhibitory or excitatory reaction. Results： 1 ） A total of 278 NTS neurons were recorded in the present study. Among them, 132 neurons showed apparent excitatory or inhibitory responses to gastric distention via the implanted balloon and were called as stomach-related neurons. Among the 132 neurons, 70 were excited, and 62 were inhibited, including 36/62 being inhibited completely. 2） Of the 132 stomach-related neurons （ 17 inhibitory and 24 excitatory neurons）, 14/17 and 8/24 presented inhibitory response, and 3/17 and 16/24 excitatory response to EA of ＂Hegu＂ （LI 4） ; while 11/17 and 7/24 presented inhibitory response, 6/17 and 17/24 excitatory response to EA of ＂Sibai＂ （ST 2） separately, indicating that signals of EA of LI-4 and ST-2 and those from gastric dilation could converge to the same NTS neuron. Conclusion： The results indicate that information of EA of ＂Sibai＂ （ST 2） and ＂Hegu＂ （LI 4） and ga

关 键 词：电针“合谷”穴“四白”穴 胃 孤束核 

分 类 号：R224.2[医药卫生—中医基础理论]