烟曲霉醇联合健择抑制人肺腺癌细胞和脐静脉内皮细胞VEGF及其受体的表达  

TNP-470 combined with Gemcitabine inhibits the expression of vascular endothelial cell growth factor and its receptors in human pulmonary adenocarcinoma cells and umbilical vein endothelium cells

作　　者：屠玲芳[1] 王雪芬[1] 安舟[1] 姚航平[1] 周建英[1] 

机构地区：[1]浙江大学医学院附属第一医院呼吸内科,杭州310003

出　　处：《浙江医学》2006年第2期81-84,共4页Zhejiang Medical Journal

基　　金：浙江省科技计划项目(2002A034)

摘　　要：目的研究烟曲霉醇(TNP-470)联合健择(Gem)对人肺腺癌细胞(A549)和人脐静脉内皮细胞(ECV-304)血管内皮生长因子(VEGF)及其受体FLT-1、KDR表达的抑制作用。方法不同浓度的Gem(10-6～10-4mgml)和TNP-470(10-3mgml)单用或联合处理A549和ECV-304细胞,用四甲基偶氮唑盐(MTT)比色法检测药物对细胞的毒性及增殖的影响,用半定量RT-PCR法和蛋白免疫印迹法(Western-blot)检测药物对细胞VEGF及其受体FLT-1和KDR的基因及蛋白表达的影响。结果Gem和TNP-470对两种细胞的增殖活性均有抑制作用(Gem的IC50为10-4mgml;TNP-470的IC50为10-2mgml),Gem联合TNP-470能明显抑制两种细胞VEGF及受体的mRNA和蛋白表达水平。结论TNP-470联合Gem能显著抑制A549、ECV-304细胞的VEGF、FLT-1和KDR的核酸和蛋白的表达,具有协同抗肿瘤作用。Objective To investigate the effect of TNP-470 and Gemcitabine（Gem） on the expression of VEGF, FLT-1, KDR in human pulmonary adenocarcinoma A549 cells and umbilical vein endothelium ECV-304 cells. Methods The proliferation of A549 and ECV-304 cells were measured by MTT. Semiquantitative RT- PCR and Western blot were used to examine the gene and protein expression of VEGF, FLT-1 and KDR after treating the cells with Gem and/or TNP-470 in different concentrations. Results The proliferation of A549 and ECV-304 were inhibited by Gem and TNP-470 （IC50 of Gem：10^-4ng/ml, IC50 of TNP-470：10^-2mg/ml）, TNP-470 combined with Gem inhibited mRNA and protein expression of VEGF, FLT-1 and KDR markedly （P〈0.05）. Conclusion TNP- 470 combined with Gemcitabine can inhibit the growth of pulmonary adenocarcinoma cells and umbilical vein endothelium cells, and their expression of VEGF, FLT-1 and KDR.

关 键 词：肺癌 烟曲霉醇 健择 血管内皮生长因子及其受体 

分 类 号：R734.2[医药卫生—肿瘤]