经典型蛋白激酶C在人卵母细胞成熟及受精过程表达研究  

Expression of classical protein kinase C in the process of maturation and fertilization of human oocyte

作　　者：武学清[1] 李晓红[1] 张晓[1] 郐艳荣[1] 王嵩[1] 程慧虹[1] 郭应禄[2] 

机构地区：[1]北京大学第一医院生殖与遗传中心 [2]北京大学泌尿外科研究所北京大学第一医院泌尿外科,北京100034

出　　处：《生殖医学杂志》2006年第1期16-19,共4页Journal of Reproductive Medicine

基　　金：国家"973"重大基金资助项目(001cb51010201)

摘　　要：目的通过检测经典型蛋白激酶C(cPKC)在人各期卵母细胞及受精卵中分布及转位,探讨cPKC在人卵母细胞成熟及受精中的作用机制。方法采用免疫荧光法检测cPKC在人GV、MII期卵母细胞、受精卵中的分布及转位。结果在人GV、MII期卵母细胞中,PKCα、βⅠ和γ均有表达。在受精后的卵母细胞中,PKCγ分布没有明显改变,PKCα转移至细胞外周,PKCβⅠ转移至染色质周围,与两个染色质没有重叠。结论PKCα、βⅠ和γ在人卵母细胞中存在,并且参与卵子受精后事件。Objective： To study the mechanism of human oocyte development and fertilization by detecting the distribution and translocation of classical protein kinase C （PKC） in human oocytes and zygotes. Methods： The subcellular localization of classical PKCα、βⅠ and γ in human GV, MⅡ oocytes and zygotes was investigated by immunocytochemistry. Results：PKCα、βⅠ and γ were evenly expressed in human oocytes. In zygote, PKCα was translocated to the periphery of the oocyte, PKCβ Ⅰ translocated around the nucleus. Conclusion： The presence and translocation of PKCα、βⅠand γ in human oocytes and zygotes suggest that PKC might play a role in the fertilization.

关 键 词：人卵母细胞 受精 蛋白激酶C 

分 类 号：R714.13[医药卫生—妇产科学]