X射线照射诱导鼻咽癌细胞hMSH2的表达  

X-irradiation Induces hMSH2 Expression in Nasopharyngeal Carcinoma Cells

作　　者：范凯[1,2] 王辉[3] 王彦[1] 富晶[1] 刘敏[1] 吕申[1] 

机构地区：[1]大连医科大学附属二院实验中心,116027 [2]大连医科大学解剖学教研室 [3]大连医科大学附属二院放疗科

出　　处：《肿瘤防治研究》2006年第2期94-96,共3页Cancer Research on Prevention and Treatment

基　　金：中国科学院知识创新工程领域前沿基金资助项目(DICPkaaaabc)

摘　　要：目的研究X射线照射对鼻咽癌CNE-1细胞错配修复基因hMSH2表达的影响,探讨放射损伤后肿瘤细胞的DNA修复机制。方法应用逆转录-PCR(RT-PCR)、免疫细胞化学及蛋白免疫印迹(Western blot)方法,检测X射线照射后对照组和实验组(照射总剂量分别为0Gy和10Gy)细胞中hM-SH2基因mRNA及蛋白表达。结果实验组细胞hMSH2基因mRNA及蛋白表达在照射终止后逐渐上调,其表达较对照组显著增强(P<0.01)。结论X射线照射可诱导鼻咽癌细胞hMSH2的表达,有助于放射损伤后肿瘤细胞DNA修复,这可能是肿瘤放疗敏感性降低的原因之一。Objective To study the effect of X-irradiation on hMSH2 expression in nasopharyngeal carcinoma（NPC） CNE-1 cells, and explore mechanisms of DNA mismatch repair after irradiation. Methods Control and experimental cells were irradiated with 0Gy and 10Gy respectively,and hMSH2 expression was tested by RT-PCR, immunocytochemistry and Western blot. Results The expression of hMSH2 mRNA and protein in experimental cells were increased after irradiation, and were significantly higher than those in control cells（P〈0. 01）. Conclusion X-irradiation may induce hMSH2 expression in NPC cells, which contributes to DNA repair and maybe responsible for reduction of radio-sensitivity after irradiation.

关 键 词：X射线 鼻咽癌 HMSH2 RT-PCR 免疫细胞化学 

分 类 号：R730.55[医药卫生—肿瘤] R739.63[医药卫生—临床医学]