TLR9基因多态性与肺癌易感性的关系  

TLR9 Gene Polymorphism and Susceptibility of Lung Cancer

作　　者：邹立君[1] 张合喜[2] 王友洁[1] 吕斌[1] 周宜开[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院,武汉430030 [2]新乡医学院预防医学教研室,河南新乡453003

出　　处：《环境与职业医学》2006年第1期8-10,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金重大项目(编号:39990570)

摘　　要：[目的]研究TLR9基因多态性与肺癌易感性的关系。[方法]采用病例一对照研究，使用PCR—SSCP（single strand conformation polymorphism）方法检测了82例非小细胞性肺癌（病例组）和82例健康对照人群（对照组）的TLR9基因-1486、-1237、1174、2848位点的单核苷酸多态性（SNP）。[结果]在164例中国湖北人群样本包括82例非小细胞性肺癌和82例正常对照中，TLR9基因-1486、1174、2848位点多态性频率在对照组和病例组的比例分别为0％和1.2％、1．2％和7．3％、0％和3．7％；-1237位点在对照组和病例组中均未发现多态性。综合分析后发现，在病例组中出现上述4个位点多态性任意一个的比例为6．7％，在对照组中为1．2％，P=0．0092，OR=11．25，95％CI1．41～90．05。164例的4个位点SNP在中国人群中的发生率分别为0．6％、0％、4.3％、1．8％，均明显低于欧裔、非裔和西班牙裔美国人的发生率。[结论]TLR9多态性在湖北人群中与肺癌有一定的关系，基因突变可能导致肺癌危险度增加。但这种联系仅仅具有边缘性的统计学显著性，且是通过多因素统计分析发现的，还需要运用更大样本量和独立因素分析的研究来探讨TLR9基因多态性与肺癌易感之间的联系。[ Objectvie ] To study the association between TLR9 gene polymorphism and lung cancer susceptibility. [ Methods ] -1468, -1237, 1174 and 2848 SNPs of TLR9 gene were screened by PCR-SSCP in 82 NSCLC （ non-small cell lung cancer ）cases and 82 matched controls. [ Results ] Among 164 blood samples in Hubei （ 82 NSCLC cases and 82 healthy controls ）, the frequencies of TLR9 gene SNPs ： the proportions of mutant at -1486, 1174 and 2848 sites between the controls and cases are 0% vs 1.2%（ P 〉 0.05 ）; 1.2% vs 7.3%（ P 〉 0.05 ）; 0% vs 3.7%（ P 〉 0.05 ）, respectively. At -1237 site of TLR9 gene, we did not detect polymorphism in the controls and cases. The occurrence of any one of 4 SNPs in the cases is 6.7% vs 1.2% in the controls （ P = 0.0092, OR = 11.25, 95% CI 1.41-90.05 ）. The incidences of these four SNPs in 164 Hubei samples are 0.6%, 0%, 4.3% and 1.8%, They are significantly lower than those in African American, European American and Hispanic American population. [ Conclusion ] Association between TLR9 polymorphism and lung cancer has leen found in Chinese population Hubei. The mutations of TLR9 gene could increase the risk of lung cancer.However, the association is only with marginally statistically significance under multiple statistical tests, and requires independent verification.

关 键 词：TLR9基因 基因多态性 肺癌 肿瘤易感性 

分 类 号：R734.2[医药卫生—肿瘤] R73-31[医药卫生—临床医学]