胆源性慢性胰腺炎动物模型建立初步研究  

Preliminary study on establishment of chronic biliary pancreatitis model in rats

作　　者：寇毅[1] 李延青[1] 孟敏[1] 马丽丽[2] 石焕[1] 任洪波[1] 傅廷亮[1] 阎明先[1] 

机构地区：[1]山东大学齐鲁医院消化内科,济南250012 [2]山东省立医院西院病理科

出　　处：《中华消化杂志》2006年第1期31-34,共4页Chinese Journal of Digestion

摘　　要：目的不完全结扎Wistar大鼠胆总管造成胆道不完全梗阻,观察胰腺的病理学变化,为建立胆源性慢性胰腺炎(BCP)的动物模型做初步可行性探讨。方法50只Wistar大鼠随机分为三组:空白对照组(10只)、实验对照组(10只)及模型组(30只)。模型组大鼠暴露出胆总管进入十二指肠的根部,游离后用1号可溶性肠线将1枚6号针头连同胆总管一起结扎,然后将针头抽出,关闭腹腔。实验对照组打开腹腔游离出胆总管末端即可。空白对照组不作干预。结果与对照组比较,模型组大鼠在第3天、第14天以后血胆红素、淀粉酶分别升高,21 d后逐渐出现胰腺腺泡细胞萎缩,纤维组织增生;30 d后腺体萎缩、纤维组织增生更明显;23%模型组大鼠并发胰腺假性囊肿。随着时间的延长,胶原纤维含量逐渐增加。结论不完全结扎Wistar大鼠的胆总管,30 d即可引起慢性胰腺炎,导致胰腺纤维化。胆总管的不全梗阻在BCP的发病过程中起关键的作用。Objective To explore the feasibility of establishing the model of chronic blliary pancreatitis by incompletely ligaturing common bile duct . Methods Fifty Wistar rats were randomly divided into three groups ： control group, experimental control group and model group. The rats in model group were exposed the root of common bile duct to enter duodenum using the dissolubility bowel line to ligating a needle with common bile duct, then drew out the needle, closing the abdominal cavity. The rats in experiment control group were opened the abdominal cavity and dissociated common bile duct, then closing the abdominal cavity. Results Compared with control group , the serum bilirubin and amylase of blood in model group were increased in 3 days and 14 days respectively and fibrotic proliferation of pancreatic tissues were found in 30 days . In model group, 23% of rats developed pancreatic pseudocyst. Conclusions Incompletely ligating the common bile duet of Wistar rats could induce chronic panereatitis and pancreatic fibrosis in 30 days. Strictures of common biliary duet may play a key role in ehronie.biliary panereatitis.

关 键 词：胰腺炎 胆系疾病 胆总管 

分 类 号：R-332[医药卫生]