急性和慢性束缚应激对大鼠内脏敏感性和神经内分泌的影响  

Effects of acute and chronic restraint stress on visceral sensitivity and neuroendocrine response in rats

作　　者：孙燕[1] 柳锋霖[1] 宋耿青[1] 汪步海[1] 钱伟[1] 侯晓华[1] 

机构地区：[1]华中科技大学附属协和医院消化内科,武汉430022

出　　处：《中华消化杂志》2006年第1期38-41,共4页Chinese Journal of Digestion

摘　　要：目的了解急性和慢性部分束缚应激对大鼠内脏敏感性以及神经内分泌反应的影响特点和持续时间。方法成年SD大鼠分为对照组(无束缚应激)、急性组和慢性组,通过腹壁回撤反射(AWR)评分评估应激前后大鼠内脏对结直肠扩张(CRD)的敏感性;并通过放免法检测应激前后不同时间点大鼠血浆促肾上腺皮质激素(ACTH)以及皮质酮(CORT)水平。结果①20和40 mm Hg压力扩张时,急、慢性组的AWR评分均显著高于基础水平(0 d),P<0.05;但在应激后第7天AWR评分显著下降,P<0.05。②急性组血浆ACTH和CORT水平显著高于对照组[(25.35±6.03)ng/ml比(7.24±2.97)ng/ml,(312.47±50.76)pg/ml比(97.00±23.33)pg/ml],但束缚应激7 d后,激素浓度[(11.81±5.03)ng/ml和(113.73±24.58)pg/ml]下降至基础水平。③慢性组血浆ACTH和CORT水平[(20.84±2.19)ng/ml和(200.41±78.10)pg/ml]显著高于对照组,且激素水平在束缚7 d后[(19.95±5.31)ng/ml和(162.51±47.08)pg/ml)]仍维持在高水平。结论急性和慢性束缚应激都可导致大鼠内脏敏感性增高,但作用可能是短暂的。急性束缚应激短暂显著提高血浆ACTH和CORT水平,而慢性束缚应激可能长期提高激素水平。Objective To investigate the short- and long-term effects of acute and chronic partial restraint stress （PRS） on visceral sensitivity to colorectal distention （CRD） and the neuroendocrine response in rats. Methods Male Sprague-Dawley rats were ramdomly divided into three groups including rats without PRS, with acute PRS（A-PRS）, and with chronic PRS （C-PRS）. The abdominal withdrawal reflex （AWR） score to CRD was assessed before stress, at the end of A-PRS or C-PRS, and 7 days after the first stress. The plasma levels of corticosterone （CORT） and adrenocorticotropic hormones （ACTH） at different time points were detected using radioimmunoassay method. Results （1） The AWR scores of the rats with A-PRS or C-PRS at 20 and 40 mm Hg immediately after stress significantly higher than those before stress （P〈0.05） and 7 days after the first stress （P〈0.05）. （2） Compared with those without PRS, the plasma levels of CORT and ACTH in rats with A-PRS after stress showed a significant elevation [ （ 25.35 ± 6.03） ng/ml vs. （ 7.24 ± 2.97 ） ng/ml, P〈 0. 05, and （312. 47±50.76）pg/mlvs. （97.00±23.33）pg/ml, P〈0.05]. However, the concentrations of CORT [（11.81 ±5.03）ng/ml and ACTH （113.73±24.58 pg/ml） 7 days after A-PRS respectively] returned to the baseline value. （3） The plasma levels of CORT and ACTH with C-PRS immediately after the last stress were （20.84 ± 2. 19） ng/ml and （200.41 ± 78. 10） pg/ml, significantly higher than those without PRS （P〈0.05）, and the concentration of these hormones 7 days after C-PRS remained elevated [ （ 19.95 ±5.31 ）ng/ml and （ 162.51 ±47.08）pg/ml,P〉0. 05]. Conclusions Both A- and C-PRS induce visceral hypersensitivity, but their effects are transient. A-PRS elevates transiently the plasma levels of CORT and ACTH, whereas C-PRS may have a long-term effect.

关 键 词：急性束缚应激 慢性束缚应激 内脏敏感性 促肾上腺激素释放激素 皮质酮 

分 类 号：R574[医药卫生—消化系统]