立体定向方法建立大鼠红藻氨酸颞叶癫癎模型及其脑电活动的实验研究  

Experimental study on electroencephalographic characteristic in kainic acid induced temporal lobe epilepsy model established with stereotactic technique in rats

作　　者：钱若兵[1] 傅先明[1] 喻廉[1] 牛朝诗[1] 汪业汉[1] 杨艳艳[1] 鲁明[1] 

机构地区：[1]安徽医科大学附属省立医院神经外科安徽省立体定向神经外科研究所,合肥230001

出　　处：《临床神经病学杂志》2006年第1期42-44,共3页Journal of Clinical Neurology

基　　金：安徽省教育厅自然科学研究项目(编号:2005KJ345zc;2005KJ359zc)

摘　　要：目的探讨采用立体定向技术建立大鼠颞叶癫癎模型的方法,并对发作后的脑电活动进行研究。方法用立体定向方法在20只SD大鼠的右侧海马注射红藻氨酸,用视频监测大鼠的行为学变化;在双侧海马、额叶皮质、杏仁核置入深部电极,对大鼠脑电活动进行长期记录。结果根据Racine行为学分级法,Ⅴ级6只,Ⅳ级10只,Ⅲ级3只,Ⅱ级1只;深部电极描记出起源于海马并向额叶皮质、杏仁核等传导癫疒间样波;病理变化提示长期癫疒间发作会导致海马锥体细胞逐渐缺失、坏死。结论用立体定向技术建立大鼠红藻氨酸颞叶癫疒间模型是一种可靠、经济和实用的方法。起源于海马并向额叶皮质、杏仁核等传导的癫疒间样波是颞叶癫疒间模型的神经电生理基础。Objective To explore the method for establish the temporal lobe epilepsy model in SD rats with stereotactic technique and to study the characteristic of electroencephalography （EEG） after epileptic seizure. Methods Kainic acid was injected into right hippocampus via stereotactic technique in 20 rats, and the behavior of rat was recorded by a video camera. The electrical activity changes of brain were recorded by EEG in bilateral hippocampus, amygdala and frontal lobe. Results According to Racine＇s scale, 6 rats were type Ⅴ, 10 rats were type Ⅳ ,3 rats were type Ⅲ, 1 rat was type Ⅱ. The spike wave was originated from hippocampus and spread to amygdala and frontal lobe. Pathologic changes showed that prolonged seizures induced pyramidal cell loss and death in hippocampus. Conclusions The method to establish kainic acid induced temporal lobe epilepsy model with stereotactic technique was credible, economical and useful. The spike wave originated from hippocampus was the basic feature of EEG of temporal lobe epilepsy model in rats.

关 键 词：立体定向 颞叶癫痫 红藻氨酸 脑电图 

分 类 号：R742.1[医药卫生—神经病学与精神病学]