大鼠脑出血后神经细胞凋亡和蛋白激酶B表达及β-七叶皂甙钠的干预作用  

Interference effects of β-aeacine sodium on the apoptosis and the expression of Akt in neurons after intracerebral hemorrhage in rats

作　　者：张勇[1] 丁新生[1] 高飞[1] 顾萍[1] 张化彪[1] 宋春杰[1] 邓晓宣[1] 姚娟[1] 朱国庆[2] 

机构地区：[1]南京医科大学第一附属医院神经内科,210029 [2]南京医科大学生理教研室

出　　处：《临床神经病学杂志》2006年第1期50-52,共3页Journal of Clinical Neurology

摘　　要：目的 观察大鼠脑出血后神经细胞凋亡和蛋白激酶B（AKt）的表达，探讨β-七叶皂甙钠的干预作用及机制。方法 将雄性SD大鼠随机分为正常组、假手术组、脑出血组和β-七叶皂甙钠治疗组。后3组又各分为6h、12h、1d、3d、7d组；用Ⅶ型胶原酶注入到大鼠右侧苍白球诱导脑出血模型，采用原位末端标记技术（TUNEL）和免疫组化方法检测损伤侧皮质区细胞凋亡及AKt表达的动态变化以及β-七叶皂甙钠对此的影响。结果 脑出血组TUNEL阳性细胞于脑出血后6h出现，AKt于脑出血后12h表达增多，二者均在第3d达到高峰，1周仍有表达。治疗组1d、3d、7d时间点TUNEL阳性细胞较脑出血组明显减少（P〈0．05．0．01），AKt表达较脑出血组显著增加（P〈0．05—0．01）。结论 β-七叶皂甙钠可以减少脑出血后神经细胞的凋亡，其机制可能与上调AKt表达有关。Objective To observe the apoptosis of neuron and protein kinase B （Akt） expression after intracerebral hemorrhage （ ICH ） in rats, and investigate the interference effect and mechanism of β-aeacine sodium on ICH injury. Methods All Sprague - Dawley male rats were randomly divided into normal group, pseudo -operation group, model group and therapeutic group treated with β-sodium aeacine. The latter three groups were divided into five subgroups respectively ：6 h, 12h, 1 d,3d and 7d. ICH models were performed by injection VK type collagenase into the the right globus pallidus. By the methods of in situ terminaldeoxynucleotidyl transferase- mediated dUTP nick end labeling （TUNEL） and immunohistochemistry, The dynamic changes of apoptosis and the expression of AKt protein were observed in the damaged cortex. Results TUNEL-positive cells appeared at 6h after collagenase injection in the model group and the expression of AKt increased at 12 h. They reached their peak at 3rd day, and were still present during 1 week. The numbers of TUNEL-positive cells in 1 d, 3 d, 1 week after ICH decreased markerly in treatment group than those of in model group （P〈0. 05 at ld and 3d,P〈0. 01 at 7d）, however, the number of AKt cells increased significantly （ P 〈 0. 05 at 1 d; P 〈 0. 01 at 3 d and 7d）. Conclusions β-aeacine sodium can reduce the number of apoptotic cells markerly after ICH. Up-regulation of the Akt maybe involved in mechanisms of treatment.

关 键 词：脑出血 凋亡 蛋白激酶B Β-七叶皂甙钠 

分 类 号：R743.34[医药卫生—神经病学与精神病学]