肝癌组织中脱-γ-羧基凝血酶原的测定及意义  

Quantitative measurement of des-γ-carboxy-prothrombin in cancerous and non-cancerous liver tissue and its role in hepatocellular carcinoma

作　　者：袁联文[1] 唐伟[2] 周建平[1] 幕内雅敏[2] 

机构地区：[1]中南大学湘雅二医院老年外科,湖南省长沙市410011 [2]日本东京大学附属病院肝胆胰外科

出　　处：《世界华人消化杂志》2006年第1期45-49,共5页World Chinese Journal of Digestology

摘　　要：目的:定量分析癌组织和非癌组织脱-γ-羧基凝血酶原(DCP)的浓度,探讨它们在血清DCP升高的作用及临床意义.方法:用电化学发光免疫分析法(ECLIA)定量测定41例肝癌患者血清、癌组织和非癌组织中DCP的含量.结果:癌组织DCP浓度平均为84447.7(7.1-2098623.7)mAU/g,非癌组织DCP浓度平均为888.1(0-23299.2)mAU/g.细胞膜上的DCP浓度明显高于细胞质中DCP浓度(P<0.001),癌组织DCP浓度明显高于非癌组织(4926.5vs195.2mAU/g,P<0.001).血清DCP浓度对数与癌组织DCP浓度对数(P=0.019)、非癌组织DCP浓度对数(P=0.020)均存在明显相关性,癌组织DCP浓度对数和非癌组织DCP浓度对数间也存在相关性(P=0.011).HCV感染的肝癌组癌组织和非癌组织DCP浓度均明显高于HCV感染阴性的肝癌组(6336.6vs1799.1mAU/g,248.0vs102.5mAU/g,P<0.05).门脉浸润的肝癌组癌组织DCP浓度明显高于没有门脉浸润的肝癌组(P=0.028),而肝静脉浸润组癌组织DCP浓度明显低于无肝静脉浸润组(P=0.042).伴有肝内转移的肝癌组非癌组织DCP浓度明显高于无肝内转移的肝癌组(P=0.023).结论:癌组织产生过量DCP是肝癌血清DCP的主要来源,是一预后标志物,但肝癌血清DCP浓度是癌组织和非癌组织产生DCP浓度的整体表现.AIM： To measure the levels of des-γ-carboxyprothrombin（DCP）in cancerous and non-cancerous liver tissues of hepatocellular carcinoma （HCC）quantitatively, and to investigate their roles in the elevated serum DCP and clinical significance. METHODS： The levels of DCP in serum, cancerous and non-cancerous liver tissue samples prepared from 41 patients with a single HCC nodule were measured using an electro-chemiluminescence immunoassay （ECLIA）. RESULTS： The levels of DCP in HCC tissues ranged from 7.1 to 2098623.7 mAU/g tissue weight, with a median of 81147.7 mAU/g, and the levels of DCP in non-cancer tissues ranged from 0 to 23299.2 mAU/g tissue weight, with a median of 888.1 mAU/g. The DCP levels in HCC tissue were significantly higher than those in non-cancer tissue （4 925.5 mAU/g vs 195.2 mAU/g, P 〈 0.001）. The DCP levels in cell membranes were significantly higher than those in the cytoplasm （P 〈 0.001）. The logarithm of serum DCP levels was correlated not only with that in HCC tissues （P = 0.019）, but also with that in non-cancer tissues （P = 0.020）. The logarithm of DCP levels in HCC tissues was correlated with that of DCP levels in non-cancer tissues （P = 0.011）. The DCP levels in HCC tissues and non-cancer tissues with hepatitis C virus infection were significantly higher than those in the tissues without HCV infection, respectively （6 336.6 mAU/g vs I 799.1 mAU/g, 248.0 mAU/ g vs 102.5 mAU/g, both P 〈 0.05）. The DCP levels in HCC tissues with portal vein invasion were significantly higher than those in HCC tissues without portal vein invasion （P = 0.028）. The DCP levels in HCC tissues with hepatic vein invasion were markedly lower than those in HCC tissues without hepatic vein invasion （P = 0.042）. Furthermore, the DCP levels in non- cancerous tissues with intrahepatic metastatic lesions were significantly higher than those in non-cancer tissues without intrahepatic metastatic lesions （P = 0.023）. CONCLUSION： The elevated serum DCP originat

关 键 词：肝癌 脱-Γ-羧基凝血酶原 电化学发光免 疫分析法 

分 类 号：R735.7[医药卫生—肿瘤] R446.11[医药卫生—临床医学]