茶多酚对大鼠肠缺血再灌注肺损伤的保护作用  

Protective effects of tea polyphenols on lung injury induced by intestinal ischemia reperfusion in rats

作　　者：王利[1] 吕莉[2] 韩国柱[2] 李楠[3] 

机构地区：[1]大连市友谊医院普外科,辽宁省大连市116001 [2]大连医科大学药理教研室,辽宁省大连市116027 [3]大连理工大学分析教研室,辽宁省大连市116023

出　　处：《中国临床康复》2006年第8期95-97,i0002,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察茶多酚对大鼠肠缺血再灌注肺损伤的保护作用,并了解这种作用是否有剂量依赖性。方法:实验于2004-03/07在大连医科大学药理教研室实验室进行。①取雄性SD大鼠60只,随机分为模型组、假手术组及茶多酚100.0,50.0,25.0和12.5mg/kg组。②各茶多酚组大鼠舌下静脉注射相应剂量的茶多酚,模型组、假手术组给予等容量生理盐水;20min后通过夹闭肠系膜上动脉60min、再灌注120min,建立肠缺血再灌注损伤模型,假手术组不夹闭肠系膜上动脉。③再灌120min后,各组取血及肺组织,测定血清及肺组织中超氧化物歧化酶、丙二醛和一氧化氮浓度,以及肺灌流液中蛋白质含量,光镜下观察肺组织形态学改变。结果:60只大鼠进入结果分析。①与模型组相比,茶多酚12.5,25.0,50.0和100.0mg/kg组大鼠血清中超氧化物歧化酶活力分别增加38.55%,125.37%,181.58%和185.34%(P<0.05,0.01);丙二醛浓度分别降低55.83%,63.56%,74.20%和80.61%(P<0.01);一氧化氮浓度分别降低11.66%,31.97%,43.24%和84.78%(P<0.05,0.01)。②与模型组相比,茶多酚12.5,25.0,50.0和100.0mg/kg组大鼠肺中超氧化物歧化酶活力分别增加8.12%(P>0.05),116.89%,186.24%和233.59%(P<0.01);丙二醛含量分别降低34.54%(P>0.05),72.69%,75.10%和80.72%(P<0.01);一氧化氮浓度分别降低30.25%,54.61%,92.87%和92.67%(P<0.01)。③与模型组相比,茶多酚12.5,25.0,50.0和100.0mg/kg组大鼠肺灌流液蛋白质含量分别降低55.98%,70.57%,75.03%和82.00%(P<0.01)。④镜检发现模型组肺有明显组织形态学损伤,茶多酚各组较模型组呈剂量依赖性减轻肺部改变。结论:茶多酚对肠缺血再灌注所致肺损伤有剂量依赖性的保护作用,可能与其自由基消除作用,减轻中性粒细胞聚集及活化,抑制大量一氧化氮释放有关。AIM：To observe the protective effects of tea polyphenols （TP） on injury of lung induced by intestinal ischemia reperfusion （I/R） in rats, and comprehend whether this action is on dose-dependent manner. METHODS： The experiment was conducted in the Staff Room of Pharmacology, Dalian Medical University from March to July 2004. ① 60 male SD rats were randomized into sham-operation group, model group and 100.0, 50.0, 25.0, 12.5 mg·kg^-1 TP group. ②Rats in each TP group received ranine vein injection of TP of corresponding dose, and rats in the model group and sham-operation group were administrated with normal saline of the same volume; The intestinal I/R injured model was established by reperfusing for 120 minutes following 20-minute clamping of su- perior mesenteric artery; Rats in the sham-operation group received no clamping of superior mesenteric artery： ③ After 120-minute reperfusion, blood and lung samples were got in all groups. The concentration of superoxide dismutase （SD）, malonaldehyde （MDA） and nitrogen oxide （NO） in serum and lung samples as well as protein content in lung perfusate were determined. Morphological changes of lung were observed under light microseope. RESULTS： A total of 60 rats entered the final analysis. ① Compared with model group, SD activity in serum of rats in the 12.5,25.0,50.0 and 100.0 mg/kg TP group increased 38.55%,125.37% ,181.58% and 185.34%（P 〈 0.05,0.01） respectively, the concentration of MDA decreased 55.83% ,63.56% ,74.20% and 80.61% （P 〈 0.01） respectively, the concentration of NO decreased 11.66% ,31.97% ,43.24% and 84.78% （P 〈 0.05, 0.01） respectively. ② In comparison with the model group, SOD activity of rats in the 12.5,25.0,50.0 and 100.0 mg/kg TP group increased 8.12%（P 〉 0.05）, 116.89%, 186.24% and 233.59% （P 〈 0.01） respectively, the content of MDA decreased 34.54%（P 〉 0.05）,72.69% ,75.10% and 80.72%（P 〈 0.01） respectively, the concentration of NO decreased 30.25% ,54.61%, 92.87%

关 键 词：再灌注损伤 丙二醛 超氧化物歧化酶 一氧化氮 茶多酚 肠缺血 保护作用 

分 类 号：R563[医药卫生—呼吸系统]