RCAS1在多种肿瘤细胞系中的表达  

Expression of RCAS1 in Different Tumor Cell Lines

作　　者：韩源[1] 黄钢[2] 

机构地区：[1]上海交通大学医学院仁济医院核医学科,上海200001 [2]上海交通大学医学院临床核医学研究所

出　　处：《上海交通大学学报（医学版）》2006年第2期142-145,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30470497);上海市科委重点基金(02DJ04137)资助项目

摘　　要：目的采用多种方法检测多种肿瘤细胞系中S iSo细胞表达的受体结合肿瘤抗原(RCAS1)的表达情况。方法采用实时定量聚合酶链反应(PCR)、Northern b lot检测、免疫组化法和酶联免疫吸附实验对10个人肿瘤细胞系及2个正常人细胞系RCAS1分子的表达进行了检测。结果实时定量PCR及Northern b lot结果显示,除了正常肝细胞LO2,各肿瘤细胞及正常人胚肾细胞293均见RCAS1 mRNA表达;免疫组化见各肿瘤细胞和293细胞的细胞膜和细胞浆中均有RCAS1蛋白,但在LO2细胞中未见RCAS1蛋白;除了HuH-7和LO2细胞,其余10个细胞培养上清液中的RCAS1蛋白量均明显高于DMEM全培液(P<0.001)。结论RCAS1广泛表达于多种肿瘤细胞系中。Objective To investigate the expression of RCAS1, which is a receptor-binding cancer antigen expressed on SiSo cells in many tumor cell lines. Methods Using real-time polymerase chain reaction （ PCR）, Northern blot, immunohistochemical examination and enzyme-linked immunosorbent assay, we detected the expression of RCAS1 in 10 different tumor cell lines and two normal cell lines. Results The expression of RCAS1 in the tumor cell lines and the fetal renal cell line （293 cell） was detected by real-time PCR and Northern blot, but not in the normal liver cell line （ LO2 cell）. The results of immunohistochemical examination on the tumor cell lines and the 293 cell showed that RCAS1 existed in cytoplasma and on cell surface. Except HUH-7 cell and LO2 cell, the soluble RCAS1 protein in supematants of the other cells was significantly more than that in DMEM （P 〈 0. 001 ）. Conclusion RCAS1 was extensively expressed in many different tumor cell lines.

关 键 词：RCAS1 表达 肿瘤细胞系 检测 受体结合肿瘤抗原 

分 类 号：R730.3[医药卫生—肿瘤]