乙肝病毒X蛋白增加HepG2细胞ras表达  

The Analysis of the Expression for the Level of ras in HepG2 Cells Enhanced by HBV X Protein

作　　者：谢怡[1] 唐承薇[2] 王春晖[3] 

机构地区：[1]重庆医科大学生殖医学系生殖医学研究室,重庆市400016 [2]重庆医科大学第一医院消化内科 [3]四川大学华西医院人类疾病生物治疗教育部重点实验室,人类疾病相关多肽研究室

出　　处：《中国肿瘤临床》2006年第4期202-204,共3页Chinese Journal of Clinical Oncology

基　　金：国家杰出青年科学基金项目资助(编号:39725012)

摘　　要：目的:研究转染乙肝病毒X基因后HepG2肝癌细胞H-ras表达的变化,以及拉米夫定是否能抑制其表达。方法:采用细胞免疫化学法、Westernblot技术检测乙肝病毒X蛋白对HepG2细胞以及核苷类似物拉米夫定对转染有乙肝病毒X基因的HepG2x细胞H-ras表达的影响。结果:X蛋白能增加HepG2细胞胞浆内H-ras表达,其面积光密度值较转染前明显增加(P<0.01),4.36×10-4mol/L拉米夫定能降低HepG2x细胞H-ras蛋白表达,其蛋白条带面积灰密度值明显低于对照(P<0.01),但拉米夫定不影响HepG2细胞H-ras表达(P>0.01)。结论:X蛋白上调肝癌细胞H-ras表达,提示可能促进HepG2细胞恶性转化。拉米夫定能抑制H-ras表达,提示拉米夫定可能遏制X蛋白的致癌作用。Objective： To study the effect of HBV X protein on the,expression of oncogene H-ras in HepG2 cells, and whether lamivudine could affect the expression of oncogene H-ras in HepG2x cells transfected with HBV X gene. Methods： The effect of X protein on the expression of H-ras in HepG2 cells and the effect of lamivudine on the HepG2x HCC cells, transfected by hepatitis B virus, were detected using immunocytochemistry and western blot. Results： X protein could increase the H-ras expression in cellular plasma of HepG2 cells. The optical density value of the area of HepG2x cells was markedly higher than that of HepG2 cells, P〈0.01, The average optical density value showed similar resuit. Lamivudine treated group at a concentration of 4.36×10^-4 mol/l, decreased H-ras expression in HepG2x cells resulted in the gray density value of the area of positive protein band was lower than that of control group, P〈0.01. But lamivudine didn＇t have any effect on HepG2 cells. Conclusion： The up-regulation of H-ras in HepG2x cells indicated an enhanced malignancy of the cells. Lamivudine could inhibit the expression of H-ras that denoted lamivudine may prevent the carcinogenesis of HBV X protein.

关 键 词：肝细胞癌 乙型肝炎病毒X蛋白 H—ras 核苷类似物拉米夫定 

分 类 号：R735.7[医药卫生—肿瘤]