颞下颌关节盘前移位后双板区组织软骨化生机制的实验研究  

Experimental study of the mechanism of chondroid metaplasia of the bilaminar zone following anterior disc displacement of temporomandibular joint

作　　者：张银凯[1] 谷志远[2] 胡勤刚[1] 

机构地区：[1]南京大学医学院附属口腔医院口腔颌面外科,江苏南京210008 [2]浙江大学医学院附属口腔医院口腔颌面外科,浙江杭州310006

出　　处：《上海口腔医学》2006年第1期48-51,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(3041900);浙江省科技厅基金(2004201)

摘　　要：目的:探讨颞下颌关节盘前移位后双板区内化生软骨细胞的来源。方法:将12只日本成年大耳白兔随机分为A、B两组,每组包括4只实验兔和2只对照兔,实验兔的右侧关节盘被手术前移并固定在前方的颧弓上,A组用于HE染色和抗增殖细胞核抗原(PCNA)、抗成纤维细胞生长因子受体3(FGFR3)免疫组化检测,B组用于透射电镜观察。结果:所有实验组动物的关节盘双板区内均出现了软骨细胞化生,部分化生软骨细胞内PCNA和FGFR3均为阳性。超微结构观察可见:部分细胞具有成纤维细胞和软骨细胞的双重特征,部分软骨细胞具有幼稚细胞的特征。结论:颞下颌关节盘前移位后,双板区内化生的软骨细胞可能部分由间充质干细胞增殖分化而来。PURPOSE： To investigate the source of the metaplasia chondrocytes in the bilaminar zone following anterior disc displacement （ADD） of temporomandibular joint （TMJ）. METHODS： 12 Japanese adult rabbits were randomly divided into A and B groups. Each group contained 4 experimental and 2 control rabbits. The disc of the experimental rabbits was induced forward and fixed into the holes made in the zygomatic arch. Group A was stained by HE and examined for the expression of proliferating cell nuclear antigen （PCNA） and fibroblast growth factor receptor 3 （FGFR3） by immunohistochemical methods. Group B was observed under transmission electron microscopy （TEM）. RESULTS： Chondrocytes appeared in the bilaminar zone of each experimental rabbit. Both PCNA and FGFR3 were positive in part of them. Under TEM, some cells took on the double characters of chondrocyte and flbroblast and some others appeared the characters of naive cells. CONCLUSIONS： Following ADD of TMJ, some metaplasia chondrocytes might be proliferated and differentiated from mesenchymal stem cells. Supported by National Natural Science Foundation of China （Grant No. 3041900） and Research Fund of Science and Technology Bureau of Zhejiang Province （2004201）.

关 键 词：颞下颌关节 关节盘前移位 双板区 软骨细胞化生 兔 

分 类 号：R782.6[医药卫生—口腔医学]