成人成牙本质细胞原位培养的初步研究  

Preliminary study on adult odontoblast culture in situ

作　　者：王晓春[1] 梁景平[1] 李昊妍[1] 张秀丽[2] 宋爱梅[1] 陈颖[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔医学院口腔内科 [2]上海市口腔医学研究所上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2006年第1期88-92,共5页Shanghai Journal of Stomatology

摘　　要：目的:建立成人成牙本质细胞原位培养模型。方法:取20～30岁成人新鲜、健康、完整的第三磨牙30颗,随机分为拔髓实验组、有血清培养组和无血清培养组,每组各10颗。冠根分离,拔髓,将成牙本质细胞保留在牙冠上,进行有血清和无血清的原位培养,每天换液,连续培养7d。通过光镜检测细胞保留情况,扫描电镜检测细胞形态和分布特征,台盼蓝检测细胞活性,对模型进行鉴定。结果:室温拔髓后,成牙本质细胞保留在髓室壁上,在7d的培养过程中细胞有活性,形态保持良好。结论:采用有血清和无血清培养,均可建立成人成牙本质细胞培养模型。PURPOSE： To establish an in situ culture model of adult odontoblasts. METHODS： Thirty intact and healthy third molars freshly prepared from 20-30 year old individuals were randomly divided into three groups.Each group had 10 molars.Group 1 was pulp tissue extraction group.Group 2 was serum-containing culture group.Group 3 was serum-free culture group. The root was dissected from the crown and the pulp was pulled out to make odontoblasts remaining in the crown. The odontoblasts were cultured in situ either in medium containing serum or serum-free medium for up to 7 days. The growth status of the cells was examined by light microscopy and cell morphology and distribution was analyzed by scanning electron microscopy. Cell viability was determined by trypan blue staining. RESULTS： After pulp removal at room temperature, odontoblasts remained in the wall of the pulp chamber, and kept viable and good morphology during the 7-day culture. CONCLUSION： We have successfully established an in situ culture model of adult primary odontoblasts in either serum-containing or serum-free medium.

关 键 词：成牙本质细胞 拔髓实验 有血清培养 无血清培养 原位培养 

分 类 号：R78[医药卫生—口腔医学]