Chfr基因在急性白血病骨髓细胞中表达的研究  

Analysis of Expression of Mitosis Checkpoint Gene Chfr in Bone Marrow Cells of Acute Leukemia Patients

作　　者：龚辉[1] 刘文励[1] 周剑峰[1] 冉丹[1] 徐慧珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液科,武汉430030

出　　处：《中国实验血液学杂志》2006年第1期31-34,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金项目(编号30271472);国家重点基础研究发展规划"973"(编号2002CB513100)

摘　　要：为了探讨急性白血病(AL)患者骨髓细胞中有丝分裂早期检查点Chfr基因的表达及其意义,对46例初诊未治疗的AL患者骨髓单个核细胞,用RT-PCR和免疫组织化学的方法检测Chfr基因mRNA及蛋白的表达,并与正常人骨髓单个核细胞进行对照。结果表明:免疫组织化学和RT-PCR分析显示,28例急性髓细胞白血病(AML)中的15例,18例急性淋巴细胞白血病(ALL)中的13例骨髓单个核细胞Chfr基因mRNA和蛋白的表达与正常对照相比显著下降,同时Chfr基因表达异常病例染色体检查存在核型异常。结论:急性白血病骨髓细胞有丝分裂检查点Chfr基因表达受损,可能在白血病发病中具有重要作用。This study was purposed to investigate the significance of mitosis checkpoint gene chfr expression in acute leukemia （AL）. 2 ml of bone marrow were extracted from each of 46 AL patients and 10 normal donors as control and their mononuclear cells were isolated. Then, their chfr expression was detected by using RT-PCR and immunohistochemistry. Normal control blood samples were also analyzed. The results showed that in 15 out of 28 cases of acute non-lymphocytic leukemia and 13 out of 18 cases of acute lymphocytic leukemia expression of chfr gene mRNA and protein significantly decreased as compared with control. The cytogenetic analysis of patients with a decreased Chfr expression revealed abnormal chromosome. In conclusion, Chfr gene is a leukemia-related gene and may play an important role in leukemia pathogenesis.

关 键 词：急性白血病 有丝分裂检查点 CHFR基因 骨髓细胞 

分 类 号：R733.71[医药卫生—肿瘤]