督脉电针与神经干细胞移植联合应用促进SCI横断大鼠前角运动神经元存活及减轻后肢肌萎缩的研究  

The combination of Goremor vessel electroacupuncture and neural stem cells transplantation to promote the survival of injured motoneuron of anterior horn and to reduce the myatrophy of hind limbs in rat spinal cord transected completely

作　　者：李晓滨[1] 曾园山[1] 陈玉玲[2] 陈穗君[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室神经科学研究室 [2]中山大学附属一院针灸科

出　　处：《中国康复医学杂志》2006年第2期104-107,共4页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金(30472132);广东省中医药管理局科研基金(101139和303013)

摘　　要：目的:探讨督脉电针与神经干细胞移植联合应用对大鼠脊髓全横断损伤的前角运动神经元存活以及减轻后肢肌萎缩有无促进作用。方法:将正常组、对照组、督脉电针组(电针组)、神经干细胞移植组(NSCs组)和督脉电针+神经干细胞移植组(电针NSCs组)成年大鼠T10脊髓段做全横断损伤,其中NSCs组和电针NSCs组在损伤处移植神经干细胞,电针组和电针NSCs组在术后开始接受督脉电针治疗。所有实验动物在脊髓损伤后存活65天。结果:电针NSCs组大鼠其腰段脊髓受损伤运动神经元的存活数量明显多于其他实验对照组大鼠。电针NSCs组大鼠后肢的股二头肌萎缩程度明显小于其他实验对照组大鼠。结论:督脉电针与神经干细胞移植联合应用能够促进大鼠脊髓全横断损伤后受损伤的脊髓前角运动神经元存活,以及减轻大鼠脊髓全横断损伤后瘫痪的后肢股二头肌的萎缩状况。Objective： To explore the effects of combination of Goremor vessel eleetroacupuneture and neural stem cells transplantation（NSCs） on the survival of injured motoneuron of anterior horn and the myatrophy of hind limbs in rat spinal cord transeeted completely. Method： Completely transection the T10 spinal cord of adult rats was performed in the normal group, the control group, the NSCs implant group （NSCs group）, the Goremor vessel electroaeupuneture group （EC group） and the Goremor vessel eleetroaeupuneture plus NSCs grafted group （EC＋NSCs group）. NSCs were simutaneously implanted into the transeeted site of spinal cord in NSCs group and EC＋NSCs group. The animals of EC group and EC＋NSCs group were treated by Goremor vessel eleetroaeupuncture after operation. All the experimental rats were fed for 65 days. Resalt：1.The survival number of injured motoneuren at the lumbar segment in EC＋NSCs group of rat were more than that in the other experimental control groups. 2.The myatrophy degree of Biceps femoris muscle at the hind limbs in EC＋NSCs group of rat were obviously less than that in the other experimental eontrel groups. Conclusion：The combination of Goremor vessel eleetreacupuneture and transplantation neural stem cells may promote the survival of injured motoneuren of the anterior horn and reduce the myatrophy degree of Biceps femoris muscle at the hind limbs suffering from paralysis after rat spinal cord transeeted completely.

关 键 词：督脉 电针 神经干细胞移植 脊髓损伤 运动神经元 肌萎缩 大鼠 

分 类 号：R245[医药卫生—针灸推拿学] R651.2[医药卫生—中医临床基础]