三肽化合物酪丝缬肽对小鼠黑色素瘤细胞B16-F10侵袭和转移的抑制作用  

Inhibitory Effect of Tripeptide Compound Tyroservaltide on Invasion and Metastasis of Mouse Melanoma Cell Line B16-F10

作　　者：车绪春[1] 陆融[1] 胡景霞[1] 郑敏娜[1] 章明放[2] 王松[1] 郁春艳[1] 杨湘龙[1] 邢冬红[1] 姚智[1] 

机构地区：[1]天津医科大学免疫教研室,天津300070 [2]天津医科大学病理教研室,天津300070

出　　处：《癌症》2006年第3期275-280,共6页Chinese Journal of Cancer

基　　金：国家"863"高技术研究发展计划项目(No.2004AA2Z3170;No.2005AA2Z3D40);国家"973"基础研究前期计划项目(No.2003CCA04300);教育部重点项目(No.03007)~~

摘　　要：背景与目的:三肽化合物酪丝缬肽(tyroservaltide,YSV)对实验性肝癌具有明显抑制作用,本研究观察YSV对小鼠黑色素瘤细胞B16-F10侵袭和转移的抑制作用。方法:MTT法检测YSV对B16-F10的细胞毒作用;利用基质胶Matrigel研究YSV对细胞粘附能力的影响;用Transwell小室侵袭模型研究YSV对肿瘤细胞侵袭能力的改变;经C57/BL6小鼠尾静脉注射B16-F10细胞,建立人工肺转移模型,观察YSV对B16-F10肺转移的影响;免疫组化方法观察YSV对细胞间粘附分子(intercellularadhesionmolecule-1,ICAM-1)在肺组织中表达水平的影响。结果:100μg/mlYSV作用48h对B16-F10细胞的增殖抑制率为24.36%;作用24h对B16-F10细胞在Matrigel胶上的粘附抑制率为36.51%;10μg/mlYSV作用48h对B16-F10细胞的侵袭抑制率为36.53%;640μg·(kg·d)-1YSV抑制B16-F10的肺转移,抑制率为62.21%;YSV组ICAM-1的组织量明显少于生理盐水组。结论:YSV具有抑制B16-F10生长和侵袭转移的作用。BACKGROUND ＆ OBJECTIVE： Tripeptide compound tyroservaltide （YSV） has obvious inhibitory effect on experimental liver cancer. This study was to evaluate the inhibitory effect of YSV on the invasion and metastasis of mouse melanoma cell line B16-F10. METHODS. The cytotoxic effect of YSV on B16-F10 cells was assessed by MTT assay, and the effects of YSV on the adhesiveness and invasiveness of B16-F10 cells were determined using Matrigel and a transwell system. B16-F10 cells were injected into the tail veins of C57BL/6 mice to establish an experimental lung metastasis model, and the effect of YSV on lung metastasis was observed. The effect of YSV on the expression of intercellular adhesion molecule-1 （ICAM-1） in lung tissue was detected by immunohistochemistry. RESULTS： YSV （100 μg/ml, 48 h） inhibited the proliferation of B16-F10 cells, with an inhibitory rate of 24.36%; YSV （100 μg/ml, 24 h） inhibited the adhesiveness of B16-F10 cells to Matrigel, with an inhibitory rate of 36.51%; YSV （10 μg/ml, 48 h） inhibited the invasiveness of B16-F10 cells, with an inhibitory rate of 36.53%; YSV [640 μg·（kg·d） ^-1] inhibited lung metastasis by B16-F10 cells, with an inhibitory rate of 62.21%. The expression of ICAM-1 in lung tissue was lower in YSV group than in normal saline group. CONCLUSION： YSV can inhibit the growth, invasion, and metastasis of B16-F10 cells.

关 键 词：酪丝缬肽 B16-F10细胞 侵袭 转移 小鼠 

分 类 号：R730.5[医药卫生—肿瘤]