应用激光捕获显微切割技术对肝细胞癌8号染色体短臂杂合性缺失的研究  

Loss of heterozygosity analysis on chromosome 8p in hepatocellolar carcinoma based on laser capture microdissection

作　　者：逄锦忠[1] 钦伦秀[1] 任宁[1] 叶青海[1] 荚卫东[1] 刘银坤[1] 汤钊猷[1] 

机构地区：[1]复旦大学肝癌研究所中山医院教育部癌变与侵袭原理重点实验室,上海200032

出　　处：《中华实验外科杂志》2006年第3期284-287,共4页Chinese Journal of Experimental Surgery

基　　金：国家杰出青年科学基金资助项目(30325041)国家科技攻关计划资助项目(2002BA711A02-4)

摘　　要：目的探讨8p杂合性缺失(LOH)的特点及其与肝细胞癌(HCC)临床病理特征的相关性。方法选择8p上5个具有高度多态性的微卫星标记。对62例HCC组织利用激光捕获显微切割(LCM)技术进行LOH分析。结果有56．5％(35／62)的HCC患者在1个或多个基因位点发生LOH。LOH频率最高的3个位点依次为D8S298(51．1％,24／47)、DSS1771(48．8％,21／43)和 D8S264(43．5％,20／46)。D8S298位点肝内转移者的LOH频率明显高于无转移者(尸<0．05);在 DSS1771位点,肿瘤直径>3 cm的LOH频率明显高于≤3 cm组(P<0．05)。结论HCC在染色体 8p特定位点上LOH明显,在这些区域可能存在一个或多个与HCC发生发展相关的肿瘤抑制基因。8p上部分位点的LOH与临床病理特征有一定的相关性。Objective To explore the features of loss of hetemzygosity （LOH） on 8p and their association with clinicopathological characteristics of hepatocellular carcinoma （HCC）. Methods Five high-polymorphic microsatellite markers located at 8p were selected to detect the LOH in 62 HCC tissues by laser capture microdissection. Results LOH at one or more loci was observed in 35 of 62 HCC patients （56.5%）. The three most frequently altered loci were D8S298 （51.1%, 24/47）, D8S1771 （48.8 96,21/43） and D8S264 （43.5 96,20/46）, respectively. LOH at D8S298 was more frequently detected in tumors with intrahepatic metastasis than those without metastasis （P〈 0.05）. LOH at D8S1771 was more frequent in tumors larger than 3 cm in size （P〈 0.05）. Conclusion LOH at specific loci on 8p was obvious in HCC, and one or more putative tumor suppressor genes located at these regions may confer a tumor growth advantage and contribute to the progression of HCC. LOH at some specific loci on 8p is associated with certain clinicopathological parameters of HCC.

关 键 词：激光 显微切割 癌.肝细胞 染色体 

分 类 号：R735.7[医药卫生—肿瘤]