体温变化对正常大鼠视网膜电图的影响  

Effect of body temperature on the electroretinogram of normal SD rats

作　　者：丁振强[1] 张作明[1] 顾永昊[1] 郭群[1] 李莉[1] 

机构地区：[1]西安第四军医大学航空航天医学系航空临床医学教研室,陕西西安710032

出　　处：《眼视光学杂志》2006年第1期29-31,35,共4页Chinese Journal of Optometry & Ophthalmology

基　　金：国家自然科学基金资助项目(30371517)

摘　　要：目的研究体温变化对正常SD大鼠视网膜电图的影响。方法12周龄SD大鼠25只,随机分为5组,每组5只。应用精密温度计测量SD大鼠肛温。实验1:大鼠体温维持在(35±1)℃,每间隔10min测量一次视网膜电图。实验2:从35℃、30℃、25℃到20℃依次降低大鼠体温,记录每个体温梯度的视网膜电图。结果实验1:视网膜电图的a波、b波和振荡电位潜伏期和幅值没有明显改变。实验2:随着体温的降低,视网膜电图的幅值明显降低、潜伏期显著延长。结论体温变化明显影响视网膜电图的潜伏期和幅值。应用视觉电生理技术评价视网膜功能时,尤其是应用小动物(例如大鼠)进行实验研究时,维持其体温恒定非常重要。Objective To investigate ERG alterations induced by changes in body temperature in the rat. Methods Twenty-five twelve-week-old SD rats were used in the study and were divided into 5 goups （each group included 5 animals）. Rectal temperature was measured with a precise thermometer and taken as the body temperature. In experiment 1, the body temperature was mauntained at （35±1）℃, and the ERC, s elicited by a constant stimulus intensity were recoiled every 10 minutes. In experiment2, the body temperature was lowered in five steps from 3.5℃ to30 ℃, 25 ℃, and20 ℃. ERGs elicited by different stimulus intensities were recorded at each body temperate level.Results In experiment 1, the mesa amplitudes and implicit times of both the a- and b-waves and the OPs O2-wave did not change significantly. In experiment 2, the amplitude of the ERG decreased significantly with a decrease in body tempersture, and the implicit times of the ERG were prolonged with a decrease in body temperature.Conclusion Body temperature greatly affects the amplitude and timing of the ERG. Great care must be taken to maintain a body temperature as nomal as possible when using the ERG to evaluate the retina, especially in small animals such as the rat.

关 键 词：体温变化/副作用 视网膜电描记术/方法 大鼠 

分 类 号：R770.4[医药卫生—眼科]