肿瘤坏死因子-α对体外血脑脊液屏障模型通透性的影响及其机制研究  

Influence of Tumor Necrosis Factor-α on Blood Brain Barrier Permeability and Its Mechanism

作　　者：尹飞[1] 曾卫民[2] 彭镜[1] 甘娜[1] 张红媛[1] 

机构地区：[1]中南大学湘雅医院儿科 [2]中南大学湘雅医学院生化系,长沙410008

出　　处：《实用儿科临床杂志》2006年第4期226-227,共2页Journal of Applied Clinical Pediatrics

基　　金：教育部留学回国人员启动基金项目资助(教外司2005383号);湖南省自然科学基金项目资助(05JJ30173)

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)对体外血脑脊液屏障(BBB)模型通透性的影响及其调控机制。方法利用脑微血管内皮细胞与星型胶质细胞共培养建立大鼠体外BBB模型,并随机分为正常对照组、TNF-α干预组和Y-27632预处理组。采用γ计数仪检测125I-BSA的通透量观察TNF-α对血脑脊液屏障通透性的影响。结果TNF-α使体外BBB模型对125I-BSA通透量明显增加,Y-27632预处理能明显拮抗TNF-α的上述作用(P<0.01)。结论TNF-α可导致BBB通透性增高,Rho/Rhok细胞信号传导通路可能参与此过程的调控。Objective To understand the changes and possible mechanism of the blood brain barrier （131313） permeability induced by tumor necrosis factor（TNF-α） in vitro. Methods BBB model was established by coculturing allogenic brain microvessel end othelial cell （BMEC） and astrocyte（AS）. The BBB model was divided randomly into normal control group, TNF-α group and Y 27632 pretreatment group. The changes of BBB permeability were evaluated by Gamma radioim muno assay counter. Results The Gamma radioimmuno assay indicated that the marker, ^125I - BSA, across the BBB model in vitro was significantly increased after TNF-α treatment compared with control group, Y - 27632 pretreatmen could prevent the permeability of BBB induced by TNF-α（ P 〈 0.01 ）. Conclusion TNF-α can increase the BBB permeability, and the Rho/Rhok signaling pathway is involved in the regulation of BBB permeability induced by TNF-α.

关 键 词：血脑脊液屏障 通透性 肿瘤坏死因子-Α 

分 类 号：R363[医药卫生—病理学]