Anti-trxs和Bar基因共转化小麦后代植株的遗传及表型分析  

Inheritance and phenotype analysis of Anti-trxs gene and Bar gene in transgenic wheat

作　　者：任江萍[1] 尹钧[1] 王新国[1] 王振云[1] 陈焕丽 

机构地区：[1]河南农业大学

出　　处：《西南农业学报》2006年第1期10-13,共4页Southwest China Journal of Agricultural Sciences

基　　金：河南省杰出人才创新基金(12100800);河南省杰出青年基金资助项目

摘　　要：经对基因枪转化小麦品种皖麦48获得的7株再生苗的PCR、PCR-Southern杂交和除草剂涂抹叶片检测,得到了4株转基因植株,证实了硫氧还蛋白反义基因(Anti-trxs)和抗除草剂Bar基因已经整合到小麦基因组中且能够正常表达。经对T1、T2植株分析表明:Anti-trxs、Bar基因能够稳定的遗传给后代,且普遍呈3∶1的分离,其遗传符合单基因控制的孟德尔遗传规律,部分株系表现1∶1的不正常分离(阳性个体较预期的少),推测这是由于雌或雄配子之一方丢失或外源基因的结构、排列等方面发生了变化的结果。转基因植株的农艺性状与对照相比,T0代有较大变异,但在T2代已得到了回复。The Anti-trxs gene and Bar gene were co-transferred into a wheat cultivar,Wanmai48,mediated by particle bomhardment. Four transgenic wheat plants were generated. The integration and transcription of the Anti-trxs gene and Bar gene were confirmed by PCR, PCR-southern and Bialaphos resistant test, respectively. Transmission of the Anti-trxs gene and Bar gene were investigated in the following generations.The results indicated that the foreign gene had been integrated into wheat genome.The inheritance pattern was 3：1 in some T2 progeny which was consistent with Mendel＇s Law. However, some progeny showed the segregation ratio of 1 ： 1. It may be related to gene deficiency of female or male gamete or gene rearrangement. Analyzing the phenotypic characteristics of transgenic plants showed that the abnormal phenotype of To plants could be due to physiological and cultivated condition.s, and the progeny plants would tend to be the normal phenotype.

关 键 词：Anti-trxs 基因 BAR基因 转基因小麦 遗传及表型分析 

分 类 号：S512.1[农业科学—作物学]