核因子-κB及Bcl-2基因在肝外胆管癌组织中表达及意义  

The expression of NF-κB and Bcl-2 gene in human extrahepatic cholangiocarcinoma and their significance.

作　　者：张建淮[1] 赵翰林[1] 孙玉洁[2] 韩晓[2] 

机构地区：[1]南京医科大学附属第一医院普外科,江苏南京210029 [2]南京医科大学江苏省人类功能基因组学重点实验室,江苏南京210029

出　　处：《现代肿瘤医学》2006年第3期303-305,共3页Journal of Modern Oncology

摘　　要：目的研究核因子κB(NF-κB)及抗凋亡基因Bcl-2在肝外胆管癌组织中的表达及意义。方法应用免疫组织化学SABC法,检测NF-κB在48例肝外胆管癌组织及12例正常肝外胆管组织中的表达变化及抗凋亡基因Bcl-2的相应表达,并分析其与肝外胆管癌临床病理的关系。结果NF-κB在肝外胆管癌组织及正常胆管组织中的阳性表达率分别为75%、16.7%,两者有显著性差异(P<0.01);Bcl-2在肝外胆管癌组织及正常胆管组织中的阳性表达率分别为64.6%、8.3%,两者有显著性差异(P<0.01)。NF-κB的阳性表达在病理分级间有显著性差异(P<0.01)且与Bcl-2的阳性表达显著相关。结论NF-κB是一种与肝外胆管癌有关的癌蛋白,其与Bcl-2高度相关,表明NF-κB可能通过对下游抗凋亡基因表达的调控,参与了肝外胆管癌的抗凋亡过程,影响肝外胆管癌的发生发展。Objective To study NF -κB expression in extrabepatic cholangiocarcinoma tissues and its correlation with the pathological stage of cancer tissues and the expression of Bcl - 2. Methods By means of immunohistochemical SABC method, the expression of NF -κB and Bcl - 2 were compared between the extrahepatic cholangiocarcinoma tissues from 48 cases and normal bile duct tissues from 12 cases. Results The positive expression of NF -κB in the extrahepatic cholangiocarcinoma group and the control group were 75% , 16.7% respectively （ P 〈 0. 01 ）. The positive expression of Bcl - 2 were 64.6% ,8.3% respectively （ P 〈0.01 ）. The positive of NF -κB was significantly different for different pathological grades（P 〈0.01 ） and the expression of Bcl-2 was closely related to NF -κB （ r = 0. 478 ,P = 0. 001 ）. Conclusion NF-κB may be an important oncoprotein, and the abnormal expression of NF -κB is closely associated with the development of extrahepatic cholangiocarcinoma. NF -κB is closely related to Bcl - 2,which indicates that NF -κB is involved in the anti - apoptosis of extrabapotic cholangiocarcinoma cell by mediating anti -apoptosis gene.

关 键 词：肝外胆管癌 NF-ΚB 基因 BCL-2 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]