脑肿瘤干细胞与神经上皮肿瘤病理级别的相关性  

Correlation research between cancer stem cells and the pathological grades of neuroepithelial tumors

作　　者：邓永文[1] 方加胜[1] 李茗初[2] 陈风华[1] 周向阳[1] 伍军[1] 周仁辉[1] 方芳[3] 陈成[1] 卢明[1] 曾飞跃[1] 

机构地区：[1]中南大学湘雅医院神经外科,长沙410008 [2]北京友谊医院神经外科,北京100050 [3]中南大学湘雅医院心内科,长沙410008

出　　处：《中南大学学报（医学版）》2006年第1期45-51,共7页Journal of Central South University ：Medical Science

基　　金：湖南省卫生厅科研基金(B2003-016);中南大学研究生教育创新工程博士研究生学位论文创新选题(040141)

摘　　要：目的:探讨脑肿瘤干细胞(brain tumor stem cells,BTSCs)的分离、培养及鉴定的方法,研究BTSCs比例与人脑神经上皮肿瘤病理级别之间的相关性。方法:将人神经上皮肿瘤细胞接种于含生长因子的无血清培养基中,使其中的脑肿瘤干细胞增殖和传代,利用细胞免疫荧光及免疫组化法检测干细胞的特异性标记物Nestin和CD133的表达;采用免疫组化(SABC法)研究46例脑肿瘤及5例正常脑组织CD133的表达,并与肿瘤病理分级进行相关性分析。结果:可以从人神经上皮肿瘤病例中分离培养出BTSCs;BTSCs可以在体外增殖并传代,并且表达干细胞的特异性标记物Nestin和CD133;CD133在正常的脑组织中未见表达,在各级神经上皮肿瘤中均有表达,各级别肿瘤中的阳性细胞比例差异有显著性(P<0.01),CD133阳性细胞比例与肿瘤的恶性程度呈正相关(P<0.01)。结论:各种病理类型神经上皮肿瘤组织中均存在干细胞特征的细胞,CD133的表达与肿瘤级别呈正相关。Objective To explore the methods of isolation, culture and identification of brain tumor stem cells （BTSCs） in neuroepithelial tumor tissues in vitro, and to study the correlation between BTSCs and the patholorical grades of neuroepithelial tumors. Methods Tumor cells from patients underby immunocytochemistry staining, and the expression of CD133 of tumor specimen sections was detected by immunohistochemistry staining . The expression of CD133 of 46 brain tumors and 5 normal brain tissues were analysed by SABC immunohistochemical staining, and the correlation between the expression and pathological grade of the tumors was analysed. Results BTSCs from neuroepithelial tumors could be isolated and cultured, and could be generated and passaged in vitro. The expression of Nestin and CD133 could be detected in BTSCs. CD133 could be detected in neuroepithelial tumor tissues, but not in normal brain tissues. There was significant difference between the expression of CD133 and the different grades of tumors （P 〈0.01 ） , and there was a positive correlation between the expression of CD133 and the histologic grading of tumors （ P 〈 0.01 ）. Conclusion A small proportion of stem cells have the abi-lity to self-renew in human neuroepithelial tumors, and there is a positive correlation between the expression of CD133 and histologic grading of tumors.

关 键 词：神经上皮肿瘤 脑肿瘤干细胞 CD133 NESTIN 病理级别 

分 类 号：R739.4[医药卫生—肿瘤]