TGIF,MMP9和蛋VEGF白在胃癌中的表达及临床病理联系  

Expressions of TGIF,MMP9 and VEGF proteins and their clinicopathological relationship in gastric cancer

作　　者：胡忠良[1] 文继舫[1] 沈明[1] 刘英[1] 

机构地区：[1]中南大学湘雅医学院病理学教研室,长沙410078

出　　处：《中南大学学报（医学版）》2006年第1期70-74,共5页Journal of Central South University ：Medical Science

摘　　要：目的:探讨TG IF,MMP9和VEGF蛋白在胃癌中的表达及其临床病理联系。方法:运用连续切片HE染色法观察SGC-7901细胞、PcDNA3.1转染细胞和TG IF转染细胞荷瘤裸鼠瘤组织有无血管浸润和远处转移,同时以免疫组织化学法分析转移相关分子MMP2,MMP9和VEGF在裸鼠瘤组织中的表达变化,并分析TG IF,MMP9和VEGF蛋白在76例胃癌组织中的表达水平及其临床病理联系。结果:3种细胞的荷瘤裸鼠均无远处转移,SGC-7901细胞和PcDNA3.1转染细胞接种组裸鼠瘤组织有癌栓形成,但TG IF组无癌栓形成;对照组MMP9和VEGF均呈强阳性表达,表达水平明显高于TG IF组,但MMP2在3组间的表达无明显差别。胃癌组织中TG IF蛋白表达阳性,阳性率为46.1%(35/76),明显低于断端胃黏膜(78.1%)(P<0.05),且它的低表达与淋巴结转移有关(P<0.05)。MMP9表达阳性率为59.2%,明显高于断端胃黏膜(31.3%)(P<0.05),且其高表达与淋巴结转移有关(P<0.05)。VEGF蛋白的阳性表达率为56.6%,明显高于断端胃黏膜(31.3%)(P<0.05),且其表达与淋巴结转移和胃癌的浸润深度密切相关(P<0.05)。TG IF与VEGF和MMP9蛋白的表达呈负相关(P<0.05)。结论:TG IF可能通过下调VEGF和MMP9蛋白的表达抑制胃癌的转移。Objective To detect the expressions of TG-interacting factor （TGIF）, matrix metalloproteinase 9 （MMP9） and vascular endothelial growth factor （VEGF） proteins, and to analyze their clinicopathological relationship in gastric cancer. Methods Vascular invasion and remote metastasis were examined in nude mice inoculated with SGC-7901 cells, vector control cells or TGIF transfected cells via serial sections, and sequentially the expressions of MMP2, MMP9 and VEGF proteins in tumor tissues from nude mice were detected by immunohistochemical staining. At the same time, TGIF, MMP9 and VEGF proteins were examined in 76 patients with gastric carcinoma, and the relationships between the expressions of the three proteins and clinicopathological features were analyzed. Results Metastasis was not observed in nude mice inoculated with any cells. There were cancerous embolisms in nude mice tumor tissue inoculated with SGC-7901 cells and vector control cells, but not in TGIF transfected cells. The expressions of MMP9 and VEGF proteins were higher in the tumor tissues originated from SGC-7901 cells and vector control cells than that from TGIF transfected cells. The expression of MMP2 had no dis tinct difference among the tumor tissues originated from the three cells. The positive rate of TGIF protein in gastric carcinoma was 46. 1% （35/76）, obviously lower than that in surgical marginal mucosa （78.1% ） （ P 〈 0.05 ）. Low expression of TGIF protein correlated significantly with the metastasis of lymph node. The positive rates of MMP9 and VEGF proteins in gastric carcinoma were 59.2% and 56.6%, respectively, obviously higher than that in surgical marginal mucosa （31.3%） （P 〈 0.05 ）. High expressions of both MMP9 and VEGF also correlated significantly with the metastasis of lymph node. The expression level of VEGF protein was also associated with the invasion of gastric carcinoma （P 〈 0.05 ）. The expression of TGIF protein reversely correlated with that of MMP9 and VEGF proteins （ P 〈 0.05

关 键 词：TGIF 血管内皮生长因子 基质金属蛋白酶9 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]