儿茶素清除O_2^-·与·OH的能力  

Ability of catechin to eliminate O_2^-·and·OH

作　　者：何小解[1] 易著文[1] 田云[2] 卢向阳[2] 党西强[1] 莫双红[1] 杨华彬[1] 

机构地区：[1]中南大学湘雅二医院小儿肾脏病研究室湖南省小儿肾脏病临床中心,长沙410011 [2]湖南农业大学,长沙410128

出　　处：《中南大学学报（医学版）》2006年第1期138-140,共3页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(02JJY3020);湖南省教育厅重点项目(02A015)

摘　　要：目的:研究儿茶素直接捕获O.-2与.OH的能力。方法:利用微弱化学发光法检测儿茶素对O2.-与.OH的清除能力;利用自旋捕集技术验证儿茶素对O2.-与.OH的清除能力。结果:儿茶素清除O2.-与.OH的IC50分别为6.16,0.59μg/mL,明显优于甘草提取物、迷迭香提取物、葡萄籽提取物、虎杖提取物及银杏叶提取物。结论:供试的几种重要天然植物抗氧化剂中,儿茶素对O2.-与.OH的清除能力最强。Objective To investigate the eliminating ability of catechin to eliminate O·2^- and OH. Methods The ability of catechin to clear away O·2^- and OH was respectively measured by faintness chemiluminescence and spin trapping assay. Results IC50 that catechin eliminated O·2^- and · OH was 6.16, 0.59 g/mL respectively, and the eliminating ability of catechin was much stronger than that of the extract from liquorice, rosemary, grape pip, giant knotweed and ginkgo leaf. Conclusion Compared with several important natural plants of antioxidants, the eliminating ability of cathechin is the best.

关 键 词：儿茶素 化学发光 自旋捕集 抗氧化剂 

分 类 号：R285[医药卫生—中药学]